The use of a concentration step to collect urinary components separated by capillary electrophoresis and further characterization of collected analytes by mass spectrometry

Norberto A. Guzman, Maria A. Trebilcock, Juan P. Advis

Research output: Contribution to journalArticlepeer-review

159 Scopus citations

Abstract

Two methods were developed using capillary electrophoresis to concentrate urinary constituents in order to collect adequate amounts of separated analytes. The first method utilizes multiple capillaries arranged in bundles and coupled to a single capillary through a glass connector. The second method consists of the use of an analyte concentrator containing an antibody covalently bound to a solid support material. Both methods allow loading of increased amounts of samples into the capillary, and the continuous collection of purified material in nanogram-microgram quantities. The samples collected by the two methods were further analyzed by mass spectrometry.

Original languageEnglish (US)
Pages (from-to)997-1015
Number of pages19
JournalJournal of Liquid Chromatography
Volume14
Issue number5
DOIs
StatePublished - Mar 1 1991

All Science Journal Classification (ASJC) codes

  • Molecular Medicine

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