TY - JOUR
T1 - Three-Dimensional Single-Molecule Localization Microscopy in Whole-Cell and Tissue Specimens
AU - Liu, Sheng
AU - Huh, Hyun
AU - Lee, Sang Hyuk
AU - Huang, Fang
N1 - Funding Information:
The authors thank Li Fang (Purdue University) for providing the data shown in Figure 4b. S.L. and F.H. are supported by grants from the US National Institutes of Health (GM119785 to F.H.) and the US Defense Advanced Research Projects Agency (D16AP00093 to F.H.). H.H. and S.-H.L. are supported by grants from the US Department of Energy (DE-SC0019313 to S.-H.L.) and the National Science Foundation (1825433 to S.-H.L.).
Publisher Copyright:
© 2020 by Annual Reviews. All rights reserved.
PY - 2020/6/4
Y1 - 2020/6/4
N2 - Super-resolution microscopy techniques are versatile and powerful tools for visualizing organelle structures, interactions, and protein functions in biomedical research. However, whole-cell and tissue specimens challenge the achievable resolution and depth of nanoscopy methods. We focus on three-dimensional single-molecule localization microscopy and review some of the major roadblocks and developing solutions to resolving thick volumes of cells and tissues at the nanoscale in three dimensions. These challenges include background fluorescence, system-and sample-induced aberrations, and information carried by photons, as well as drift correction, volume reconstruction, and photobleaching mitigation. We also highlight examples of innovations that have demonstrated significant breakthroughs in addressing the abovementioned challenges together with their core concepts as well as their trade-offs.
AB - Super-resolution microscopy techniques are versatile and powerful tools for visualizing organelle structures, interactions, and protein functions in biomedical research. However, whole-cell and tissue specimens challenge the achievable resolution and depth of nanoscopy methods. We focus on three-dimensional single-molecule localization microscopy and review some of the major roadblocks and developing solutions to resolving thick volumes of cells and tissues at the nanoscale in three dimensions. These challenges include background fluorescence, system-and sample-induced aberrations, and information carried by photons, as well as drift correction, volume reconstruction, and photobleaching mitigation. We also highlight examples of innovations that have demonstrated significant breakthroughs in addressing the abovementioned challenges together with their core concepts as well as their trade-offs.
KW - CRLB
KW - Cramér-Rao lower bound
KW - Super-resolution microscopy
KW - adaptive optics
KW - fluorescence microscopy
KW - light-sheet microscopy
KW - tissue imaging
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U2 - 10.1146/annurev-bioeng-060418-052203
DO - 10.1146/annurev-bioeng-060418-052203
M3 - Review article
C2 - 32243765
AN - SCOPUS:85086052886
SN - 1523-9829
VL - 22
SP - 155
EP - 184
JO - Annual Review of Biomedical Engineering
JF - Annual Review of Biomedical Engineering
ER -