Three stage sample preparation for purification of proteins from complex biological samples

M. Javanmard, S. Emaminejad, R. W. Davis, C. Gupta, R. T. Howe

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Scopus citations

Abstract

In this manuscript we present a three-component sample preparation platform for delivering proteins, with controlled orientation, purified from a complex biological sample to a sensor surface. The presented platform depletes highly abundant proteins and cells from complex biological samples, and then is capable of immobilizing the target analyte on the surface with controlled immobilization. A herringbone structure is used to mix the test sample with beads coated with antibodies against the highly abundant proteins, which is then fed into a second component, consisting of a filter trench with interdigitated electrodes above for applying dielectrophoretic force. The beads and cells are pushed down into the filter trench, thus allowing only the serum to pass through. In a third step, protein orientation and immobilization is controlled with electric field. In this manuscript we discuss the proof of concept for each of these three components separately.

Original languageEnglish (US)
Title of host publicationIEEE SENSORS 2013 - Proceedings
PublisherIEEE Computer Society
ISBN (Print)9781467346405
DOIs
StatePublished - 2013
Externally publishedYes
Event12th IEEE SENSORS 2013 Conference - Baltimore, MD, United States
Duration: Nov 4 2013Nov 6 2013

Publication series

NameProceedings of IEEE Sensors

Other

Other12th IEEE SENSORS 2013 Conference
CountryUnited States
CityBaltimore, MD
Period11/4/1311/6/13

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of 'Three stage sample preparation for purification of proteins from complex biological samples'. Together they form a unique fingerprint.

Cite this