@article{539cd69872c24c42867461fa01b23a2b,
title = "Thymic nuclear matrix associated activity is not V(D)J recombinase",
abstract = "It was previously reported that nuclear matrix isolated from young rat thymus contained an activity that supported V(D)J recombination at a high efficiency (Dave et al., BIOCHEMISTRY 30: 4763-4767, 1991). A similar type of activity is also detected in the matrix prepared from fetal calf thymus. However, restriction enzyme mapping analyses of the recombined product clearly suggest that the double antibiotic resistance exhibited by the matrix treated plasmid substrate is not a consequence of V(D)J signal sequence recombination.",
author = "Pandey, {V. N.} and Dave, {V. P.} and Amrute, {S. B.} and K. Rosenbach and Modak, {M. J.}",
note = "Funding Information: V(D)J recombination is a site specific recombination that occurs in vertebrates during the process of immunoglobulin gene as well as T cell receptor gene assembly (1-3). A detailed structural analyses of the gene sequences in the embryonic and somatic life of the organism has provided some understanding of the underlying process although the biochemical mechanisms are largely unclear. The heptamer nonamer sequences separated by 12 or 23 base pair spacers appears to be the basic recognition signal sequences for the process of V(D)J gene segment joining. A number of plasmid resistant DNA substrates that contain these +Supported in part by a grant from the National Science Foundation (DMB-8715829) to M. J. M. *Address correspondence to this author.",
year = "1991",
month = nov,
day = "27",
doi = "10.1016/S0006-291X(05)81386-5",
language = "English (US)",
volume = "181",
pages = "95--99",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",
}