TY - JOUR
T1 - Thymoquinone-loaded polymeric films and hydrogels for bacterial disinfection and wound healing
AU - Haq, Anika
AU - Kumar, Suneel
AU - Mao, Yong
AU - Berthiaume, Francois
AU - Michniak-Kohn, Bozena
N1 - Funding Information:
Acknowledgments: We would like to thank Alexa Chu, Biomedical Engineering, Rutgers University for technical assistance. This work was partially supported by the New Jersey Commission on Spinal Cord Research (CSCR19ERG004 and CSCR17ERG006).
Funding Information:
We would like to thank Alexa Chu, Biomedical Engineering, Rutgers University for technical assistance. This work was partially supported by the New Jersey Commission on Spinal Cord Research (CSCR19ERG004 and CSCR17ERG006).
Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2020/10
Y1 - 2020/10
N2 - The purpose of this study was to synthesize and characterize novel biocompatible topical polymeric film and hydrogel systems that have the potential to deliver the antibacterial agent thymoquinone (TQ) directly to the skin target site to manage the local wound infection and thereby wound healing. The polyvinyl pyrrolidone (PVP) matrix-type films containing TQ were prepared by the solvent casting method. In vitro skin permeation studies on human cadaver skin produced a mean flux of 2.3 µg TQ/cm2 /h. Human keratinocyte monolayers subjected to a scratch wound (an in vitro wound healing assay) showed 85% wound closure at day 6 in the TQ group (100 ng/mL TQ) as compared to 50% in the vehicle control group (p = 0.0001). In a zone-of-inhibition (ZOI) assay, TQ-containing films and hydrogels completely wiped out Staphylococcus aureus in 10 cm diameter Tryptic Soy Agar plates while 500 µg/mL gentamicin containing filters gave 10 mm of ZOI. In an ex vivo model, TQ-containing films eradicated bacterial colonization on human cadaver skin. Furthermore, in a full-thickness wound infection model in mice, TQ-containing films showed significant activity in controlling Staphylococcus aureus infection, thereby disinfecting the skin wound. In summary, TQ-containing PVP films and hydrogels developed in this study have the potential to treat and manage wound infections.
AB - The purpose of this study was to synthesize and characterize novel biocompatible topical polymeric film and hydrogel systems that have the potential to deliver the antibacterial agent thymoquinone (TQ) directly to the skin target site to manage the local wound infection and thereby wound healing. The polyvinyl pyrrolidone (PVP) matrix-type films containing TQ were prepared by the solvent casting method. In vitro skin permeation studies on human cadaver skin produced a mean flux of 2.3 µg TQ/cm2 /h. Human keratinocyte monolayers subjected to a scratch wound (an in vitro wound healing assay) showed 85% wound closure at day 6 in the TQ group (100 ng/mL TQ) as compared to 50% in the vehicle control group (p = 0.0001). In a zone-of-inhibition (ZOI) assay, TQ-containing films and hydrogels completely wiped out Staphylococcus aureus in 10 cm diameter Tryptic Soy Agar plates while 500 µg/mL gentamicin containing filters gave 10 mm of ZOI. In an ex vivo model, TQ-containing films eradicated bacterial colonization on human cadaver skin. Furthermore, in a full-thickness wound infection model in mice, TQ-containing films showed significant activity in controlling Staphylococcus aureus infection, thereby disinfecting the skin wound. In summary, TQ-containing PVP films and hydrogels developed in this study have the potential to treat and manage wound infections.
KW - Bacterial skin infections
KW - Polymeric film and hydrogel
KW - Staphylococcus aureus
KW - Thymoquinone
KW - Topical/transdermal drug delivery
KW - Wound disinfection
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U2 - 10.3390/biomedicines8100386
DO - 10.3390/biomedicines8100386
M3 - Article
AN - SCOPUS:85093959955
SN - 2227-9059
VL - 8
SP - 1
EP - 20
JO - Biomedicines
JF - Biomedicines
IS - 10
M1 - 386
ER -