TIP 1, a cold shock-inducible gene of Saccharomyces cerevisiae

K. Kondo, M. Inouye

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Abstract

Using differential hybridization, genes whose expression is induced at low temperatures were identified in yeast Saccharomyces cerevisiae. One of these genes that corresponds to an mRNA that is induced 6-8-fold within 2 h after shifting the culture temperature from 30 to 10 °C was further characterized. Surprisingly, its expression was also induced by heat shock, and thus the gene was designated TIP1 (temperature shock-inducible protein gene). Southern hybridization analysis demonstrated that there are several genes homologous to the TIP 1 gene on the yeast genome. A TIP 1 disruption mutation exerted an observable effect neither on growth nor on viability after being exposed to freezing temperatures. The TIP 1 gene encodes a protein of 210 amino acid residues with a molecular weight of 20,727, containing 20.0% alanine and 23.3% serine. The TIP 1 protein has a typical signal peptide at the amino-terminal end and an extremely hydrophobic sequence at the carboxyl-terminal end. The TIP 1 protein is thus likely to be secreted across the membrane and anchored on the outside surface of the plasma membrane. These results indicate that the TIP 1 protein is a new type of stress-inducible protein in yeast.

Original languageEnglish (US)
Pages (from-to)17537-17544
Number of pages8
JournalJournal of Biological Chemistry
Volume266
Issue number26
StatePublished - 1991

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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