Tissue welding with virus-sterilized human cryoprecipitate

Mathew R. Williams; Christian I. Fras; Richard Moscarelli; Steven K. Libutti; Mehmet C. Oz; Lawrence S. Bass; Adrianne J. Setton; Murat Kaynar; Roman Nowygrod; Michael R. Treat

doi:10.1117/12.137351

Tissue welding with virus-sterilized human cryoprecipitate

Mathew R. Williams, Christian I. Fras, Richard Moscarelli, Steven K. Libutti, Mehmet C. Oz, Lawrence S. Bass, Adrianne J. Setton, Murat Kaynar, Roman Nowygrod, Michael R. Treat

Research output: Contribution to journal › Conference article › peer-review

1 Scopus citations

Abstract

Clinical use of laser tissue soldering with cryoprecipitate has been delayed by the fear of infecting recipients with donor viral products. Solvent-Detergent (S/D) treatment of human plasma is a technique for disrupting membrane enveloped viruses and rendering them noninfectious. Dual 6 cm incisions were created on the dorsum of nine rats and closed with either standard skin staples or with laser activated S/D cryoprecipitate. The animals were sacrificed at one of three time periods: 0, 2, and 4 days. The use of the laser tissue solder significantly improved tensile strength over standard skin closures at all time periods. Tensile strengths (kg force/sq. cm) for the laser group were: 0.22 + 0.08 (0 day), 0.77 + 0.37 (2 day), and 0.84 + 0.30 (4 day). Comparable results for the control group were significantly different (p <0.05): 0.00 (0 day), 0.12 + 0.03 ( 2 day), and 0.36 + 0.21 (4 day). Deactivation of viral particles during preparation of cryoprecipitate does not reduce the utility of this material as a solder during laser bonding. Reduced infectivity of S/D prepared products enhances their clinical utility.

Original language	English (US)
Pages (from-to)	267-270
Number of pages	4
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	1643
DOIs	https://doi.org/10.1117/12.137351
State	Published - Jun 1 1992
Externally published	Yes
Event	Laser Surgery: Advanced Characterization, Therapeutics, and Systems III 1992 - Los Angeles, United States Duration: Jan 19 1992 → Jan 24 1992

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

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10.1117/12.137351

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title = "Tissue welding with virus-sterilized human cryoprecipitate",

abstract = "Clinical use of laser tissue soldering with cryoprecipitate has been delayed by the fear of infecting recipients with donor viral products. Solvent-Detergent (S/D) treatment of human plasma is a technique for disrupting membrane enveloped viruses and rendering them noninfectious. Dual 6 cm incisions were created on the dorsum of nine rats and closed with either standard skin staples or with laser activated S/D cryoprecipitate. The animals were sacrificed at one of three time periods: 0, 2, and 4 days. The use of the laser tissue solder significantly improved tensile strength over standard skin closures at all time periods. Tensile strengths (kg force/sq. cm) for the laser group were: 0.22 + 0.08 (0 day), 0.77 + 0.37 (2 day), and 0.84 + 0.30 (4 day). Comparable results for the control group were significantly different (p <0.05): 0.00 (0 day), 0.12 + 0.03 ( 2 day), and 0.36 + 0.21 (4 day). Deactivation of viral particles during preparation of cryoprecipitate does not reduce the utility of this material as a solder during laser bonding. Reduced infectivity of S/D prepared products enhances their clinical utility.",

author = "Williams, {Mathew R.} and Fras, {Christian I.} and Richard Moscarelli and Libutti, {Steven K.} and Oz, {Mehmet C.} and Bass, {Lawrence S.} and Setton, {Adrianne J.} and Murat Kaynar and Roman Nowygrod and Treat, {Michael R.}",

note = "Publisher Copyright: {\textcopyright} 1992 SPIE. All rights reserved.; Laser Surgery: Advanced Characterization, Therapeutics, and Systems III 1992 ; Conference date: 19-01-1992 Through 24-01-1992",

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doi = "10.1117/12.137351",

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T1 - Tissue welding with virus-sterilized human cryoprecipitate

AU - Williams, Mathew R.

AU - Fras, Christian I.

AU - Moscarelli, Richard

AU - Libutti, Steven K.

AU - Oz, Mehmet C.

AU - Bass, Lawrence S.

AU - Setton, Adrianne J.

AU - Kaynar, Murat

AU - Nowygrod, Roman

AU - Treat, Michael R.

PY - 1992/6/1

Y1 - 1992/6/1

N2 - Clinical use of laser tissue soldering with cryoprecipitate has been delayed by the fear of infecting recipients with donor viral products. Solvent-Detergent (S/D) treatment of human plasma is a technique for disrupting membrane enveloped viruses and rendering them noninfectious. Dual 6 cm incisions were created on the dorsum of nine rats and closed with either standard skin staples or with laser activated S/D cryoprecipitate. The animals were sacrificed at one of three time periods: 0, 2, and 4 days. The use of the laser tissue solder significantly improved tensile strength over standard skin closures at all time periods. Tensile strengths (kg force/sq. cm) for the laser group were: 0.22 + 0.08 (0 day), 0.77 + 0.37 (2 day), and 0.84 + 0.30 (4 day). Comparable results for the control group were significantly different (p <0.05): 0.00 (0 day), 0.12 + 0.03 ( 2 day), and 0.36 + 0.21 (4 day). Deactivation of viral particles during preparation of cryoprecipitate does not reduce the utility of this material as a solder during laser bonding. Reduced infectivity of S/D prepared products enhances their clinical utility.

AB - Clinical use of laser tissue soldering with cryoprecipitate has been delayed by the fear of infecting recipients with donor viral products. Solvent-Detergent (S/D) treatment of human plasma is a technique for disrupting membrane enveloped viruses and rendering them noninfectious. Dual 6 cm incisions were created on the dorsum of nine rats and closed with either standard skin staples or with laser activated S/D cryoprecipitate. The animals were sacrificed at one of three time periods: 0, 2, and 4 days. The use of the laser tissue solder significantly improved tensile strength over standard skin closures at all time periods. Tensile strengths (kg force/sq. cm) for the laser group were: 0.22 + 0.08 (0 day), 0.77 + 0.37 (2 day), and 0.84 + 0.30 (4 day). Comparable results for the control group were significantly different (p <0.05): 0.00 (0 day), 0.12 + 0.03 ( 2 day), and 0.36 + 0.21 (4 day). Deactivation of viral particles during preparation of cryoprecipitate does not reduce the utility of this material as a solder during laser bonding. Reduced infectivity of S/D prepared products enhances their clinical utility.

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T2 - Laser Surgery: Advanced Characterization, Therapeutics, and Systems III 1992

Y2 - 19 January 1992 through 24 January 1992

ER -

Tissue welding with virus-sterilized human cryoprecipitate

Abstract

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