Transcriptional characterization of the antioxidant response of Mycobacterium tuberculosis in vivo and during adaptation to hypoxia in vitro

Lanbo Shi, Charles D. Sohaskey, Robert J. North, Maria L. Gennaro

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Transcriptional profiling of antioxidant genes of Mycobacterium tuberculosis was performed by real-time RT-PCR during mouse lung infection and during adaptation to gradual oxygen depletion in vitro. M. tuberculosis genes involved in major detoxification pathways of oxidative stress were not up-regulated during chronic mouse lung infection, which is established in response to expression of host adaptive immunity. This result suggests that a major function of bacterial antioxidant enzymes is to protect from oxidants generated during the early, acute phase of infection. In vivo transcription profiles of bacterial antioxidant enzymes differed from those seen under adaptation to low oxygen in vitro, indicating differences between growth arrest in vivo and that induced by hypoxia in vitro.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalTuberculosis
Volume88
Issue number1
DOIs
StatePublished - Jan 2008

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

Keywords

  • Bacterial growth arrest
  • Bacterial persistence
  • Mouse lung infection
  • Reactive nitrogen species
  • Reactive oxygen species
  • Reverse transcription polymerase chain reaction

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