TY - JOUR
T1 - Transcriptome-wide distribution and function of RNA hydroxymethylcytosine
AU - Delatte, Benjamin
AU - Wang, Fei
AU - Ngoc, Long Vo
AU - Collignon, Evelyne
AU - Bonvin, Elise
AU - Deplus, Rachel
AU - Calonne, Emilie
AU - Hassabi, Bouchra
AU - Putmans, Pascale
AU - Awe, Stephan
AU - Wetzel, Collin
AU - Kreher, Judith
AU - Soin, Romuald
AU - Creppe, Catherine
AU - Limbach, Patrick A.
AU - Gueydan, Cyril
AU - Kruys, Véronique
AU - Brehm, Alexander
AU - Minakhina, Svetlana
AU - Defrance, Matthieu
AU - Steward, Ruth
AU - Fuks, François
N1 - Funding Information:
We thank L. Droogmans for advice, as well as A. Rao and J. Kadonaga for continued support. We also thank Y. N. Jan for providing the antibody to Dpn. R.D., B.H., C.C., and E.B. were supported by the Belgian Fonds de la Recherche Scientifique (FNRS). B.D. was supported by the FNRS («Aspirant") and a Belgian American Educational Foundation (BAEF) fellowship. L.V.N. was supported by the Belgian Fund for Research Training in Industry and Agriculture and a BAEF fellowship. E.Co. was supported by a "l''Oréal"fellowship, and M.D. was supported by the WBHealth grant (CanDx) from the Walloon Region. F.F. is a ULB Professor. F.F.''s lab was funded by grants from the FNRS and Télévie, the Interuniversity Attraction Poles (P7/03) program, the Action de Recherche Concertée (ARC) (AUWB-2010-2015 ULB-No 7), the WB Health program, the Belgian Fondation contre le Cancer, and the Fonds Gaston Ithier. F.W. was supported by a Charles and Johanna Busch graduate fellowship and the work at Rutgers by a NIH grant (RO1 GM089992) to R.S. V.K. was supported by the ARC (AV.12/17) as well as Brachet and Van Buuren Funds. P.A.L. received financial support from the National Science Foundation (NSF CHE 1212625 and 1507357). The authors declare no conflicts of interest. All sequencing data have been deposited to the Gene Expression Omnibus as series GSE66090.
PY - 2016/1/15
Y1 - 2016/1/15
N2 - Hydroxymethylcytosine, well described in DNA, occurs also in RNA. Here, we show that hydroxymethylcytosine preferentially marks polyadenylated RNAs and is deposited by Tet in Drosophila. We map the transcriptome-wide hydroxymethylation landscape, revealing hydroxymethylcytosine in the transcripts ofmany genes, notably in coding sequences, and identify consensus sites for hydroxymethylation.We found that RNA hydroxymethylation can favor mRNA translation.Tet and hydroxymethylated RNA are found to be most abundant in the Drosophila brain, and Tet-deficient fruitflies suffer impaired brain development, accompanied by decreased RNA hydroxymethylation. This study highlights the distribution, localization, and function of cytosine hydroxymethylation and identifies central roles for this modification in Drosophila.
AB - Hydroxymethylcytosine, well described in DNA, occurs also in RNA. Here, we show that hydroxymethylcytosine preferentially marks polyadenylated RNAs and is deposited by Tet in Drosophila. We map the transcriptome-wide hydroxymethylation landscape, revealing hydroxymethylcytosine in the transcripts ofmany genes, notably in coding sequences, and identify consensus sites for hydroxymethylation.We found that RNA hydroxymethylation can favor mRNA translation.Tet and hydroxymethylated RNA are found to be most abundant in the Drosophila brain, and Tet-deficient fruitflies suffer impaired brain development, accompanied by decreased RNA hydroxymethylation. This study highlights the distribution, localization, and function of cytosine hydroxymethylation and identifies central roles for this modification in Drosophila.
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U2 - 10.1126/science.aac5253
DO - 10.1126/science.aac5253
M3 - Article
C2 - 26816380
AN - SCOPUS:84954349048
SN - 0036-8075
VL - 351
SP - 282
EP - 285
JO - Science
JF - Science
IS - 6270
ER -