Transformation and transduction in Bacillus subtilis

Evidence for separate modes of recombinant formation

David Dubnau, Rosa Davidoff-Abelson, Issar Smith

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

In Bacillus subtilis strains 168 and W23 the specificity requirements of transduction with bacteriophage PBS1 are more stringent than those of transformation. Heterologous transduction between these strains occurs at a low frequency or not at all, whereas heterologous transformation occurs readily. The heterologous transduction which does occur in this system displays the loose genetic linkage characteristic of transformation and is sensitive to a rec † Abbreviations used: rec, recombination deficient; ery, erythromycin; str, streptomycin; mic, micrococcin; leu, leucine; met, methionine; pur, adenine or guanine; arg, arginine; phe, phenylalanine; ura, uracil; trp, tryptophan; nia, niacin; ser, serine; aro, aromatic amino acids; his, histidine; tyr, tyrosine; thr, threonine; rib, riboflavin; ilv, isoleucine & valine; ile, isoleucine; amm, inability to assimilate NH4+; cys, cysteine; lys, lysine; thy, thymine. mutation which depresses transformation but hardly affects homologous transduction. It is suggested that two pathways for handling donor genetic material exist in B. subtilis. Pathway I predominates in transformation and is relatively permissive to foreign DNA, while pathway II predominates in transduction and is relatively non-permissive. The basis of the foreigness recognized in this system is most likely lack of DNA base sequence homology rather than DNA modification. Limited base-sequence inhomology is demonstrated between W23 and 168 DNA, but no evidence could be obtained for the existence of a restriction-modification system. A hypothesis is presented in which the difference in size between transforming and transducing DNA fragments is the most important factor determining the specificity difference between transformation and transduction in B. subtilis. This hypothesis is supported by heterologous transduction experiments using bacteriophage SP10, the DNA of which is smaller than that of PBS1. The level of heterologous transduction obtained with SP10 is intermediate between the level found with PBS1 and that obtained using heterologous transformation.

Original languageEnglish (US)
Pages (from-to)155-179
Number of pages25
JournalJournal of molecular biology
Volume45
Issue number2
DOIs
StatePublished - Oct 28 1969

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Bacillus subtilis
DNA
Isoleucine
Bacteriophages
Nucleic Acid Sequence Homology
DNA Restriction-Modification Enzymes
Aromatic Amino Acids
Genetic Linkage
Thymine
Uracil
Riboflavin
Niacin
Guanine
Valine
Ribs
Streptomycin
Adenine
Erythromycin
Threonine
Phenylalanine

All Science Journal Classification (ASJC) codes

  • Molecular Biology

Cite this

@article{857e72a91db94ed3bcd8afd176aae6c0,
title = "Transformation and transduction in Bacillus subtilis: Evidence for separate modes of recombinant formation",
abstract = "In Bacillus subtilis strains 168 and W23 the specificity requirements of transduction with bacteriophage PBS1 are more stringent than those of transformation. Heterologous transduction between these strains occurs at a low frequency or not at all, whereas heterologous transformation occurs readily. The heterologous transduction which does occur in this system displays the loose genetic linkage characteristic of transformation and is sensitive to a rec† † Abbreviations used: rec, recombination deficient; ery, erythromycin; str, streptomycin; mic, micrococcin; leu, leucine; met, methionine; pur, adenine or guanine; arg, arginine; phe, phenylalanine; ura, uracil; trp, tryptophan; nia, niacin; ser, serine; aro, aromatic amino acids; his, histidine; tyr, tyrosine; thr, threonine; rib, riboflavin; ilv, isoleucine & valine; ile, isoleucine; amm, inability to assimilate NH4+; cys, cysteine; lys, lysine; thy, thymine. mutation which depresses transformation but hardly affects homologous transduction. It is suggested that two pathways for handling donor genetic material exist in B. subtilis. Pathway I predominates in transformation and is relatively permissive to foreign DNA, while pathway II predominates in transduction and is relatively non-permissive. The basis of the foreigness recognized in this system is most likely lack of DNA base sequence homology rather than DNA modification. Limited base-sequence inhomology is demonstrated between W23 and 168 DNA, but no evidence could be obtained for the existence of a restriction-modification system. A hypothesis is presented in which the difference in size between transforming and transducing DNA fragments is the most important factor determining the specificity difference between transformation and transduction in B. subtilis. This hypothesis is supported by heterologous transduction experiments using bacteriophage SP10, the DNA of which is smaller than that of PBS1. The level of heterologous transduction obtained with SP10 is intermediate between the level found with PBS1 and that obtained using heterologous transformation.",
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Transformation and transduction in Bacillus subtilis : Evidence for separate modes of recombinant formation. / Dubnau, David; Davidoff-Abelson, Rosa; Smith, Issar.

In: Journal of molecular biology, Vol. 45, No. 2, 28.10.1969, p. 155-179.

Research output: Contribution to journalArticle

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N2 - In Bacillus subtilis strains 168 and W23 the specificity requirements of transduction with bacteriophage PBS1 are more stringent than those of transformation. Heterologous transduction between these strains occurs at a low frequency or not at all, whereas heterologous transformation occurs readily. The heterologous transduction which does occur in this system displays the loose genetic linkage characteristic of transformation and is sensitive to a rec† † Abbreviations used: rec, recombination deficient; ery, erythromycin; str, streptomycin; mic, micrococcin; leu, leucine; met, methionine; pur, adenine or guanine; arg, arginine; phe, phenylalanine; ura, uracil; trp, tryptophan; nia, niacin; ser, serine; aro, aromatic amino acids; his, histidine; tyr, tyrosine; thr, threonine; rib, riboflavin; ilv, isoleucine & valine; ile, isoleucine; amm, inability to assimilate NH4+; cys, cysteine; lys, lysine; thy, thymine. mutation which depresses transformation but hardly affects homologous transduction. It is suggested that two pathways for handling donor genetic material exist in B. subtilis. Pathway I predominates in transformation and is relatively permissive to foreign DNA, while pathway II predominates in transduction and is relatively non-permissive. The basis of the foreigness recognized in this system is most likely lack of DNA base sequence homology rather than DNA modification. Limited base-sequence inhomology is demonstrated between W23 and 168 DNA, but no evidence could be obtained for the existence of a restriction-modification system. A hypothesis is presented in which the difference in size between transforming and transducing DNA fragments is the most important factor determining the specificity difference between transformation and transduction in B. subtilis. This hypothesis is supported by heterologous transduction experiments using bacteriophage SP10, the DNA of which is smaller than that of PBS1. The level of heterologous transduction obtained with SP10 is intermediate between the level found with PBS1 and that obtained using heterologous transformation.

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