Transformation of the Plastid Genome to Study RNA Editing

Kerry A. Lutz, Pal Maliga

Research output: Chapter in Book/Report/Conference proceedingChapter

18 Scopus citations

Abstract

In this chapter we provide an overview of cytosine-to-uridine (C-to-U) RNA editing in the plastids of higher plants. Particular emphasis will be placed on the role plastid transformation played in understanding the editing process. We discuss how plastid transformation enabled identification of mRNA cis elements for editing and gave the first insight into the role of editing trans factors. The introduction will be followed by a protocol for plastid transformation, including vector design employed to identify editing cis elements. We also discuss how to test RNA editing in vivo by cDNA sequencing. At the end, we summarize the status of the field and outline future directions.

Original languageEnglish (US)
Title of host publicationRNA Editing
PublisherAcademic Press Inc.
Pages501-518
Number of pages18
ISBN (Print)9780123739223
DOIs
StatePublished - 2007

Publication series

NameMethods in Enzymology
Volume424
ISSN (Print)0076-6879

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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