Transformation of triploid bermudagrass (Cynodon dactylon x C. transvaalensis cv. TifEagle) by means of biolistic bombardment

G. Zhang; S. Lu; T. A. Chen; C. R. Funk; W. A. Meyer

doi:10.1007/s00299-003-0585-x

Transformation of triploid bermudagrass (Cynodon dactylon x C. transvaalensis cv. TifEagle) by means of biolistic bombardment

G. Zhang, S. Lu, T. A. Chen, C. R. Funk, W. A. Meyer

School of Environmental and Biological Sciences, Plant Biology

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

A transformation system for triploid bermudagrass (Cynodon dactylon x C. transvaalensis cv. TifEagle) was established with a biolistic bombardment delivery system. Embryogenic callus was induced from stolons and maintained on Murashige and Skoog's medium supplemented with 30 μM dicamba, 20 μM benzylaminopurine, and 100 mg/l myo-inositol. Using the hygromycin phosphotransferase (hpt) gene as the selectable marker gene, we obtained 75 transgenic lines from 18 petri dishes bombarded. Integration of the hpt gene into genomic DNA and transcription of hpt was confirmed by Southern and Northern blot analyses, respectively. Through suspension culture screening, we obtained homogeneously transformed plants showing stable transcription of the hpt gene.

Original language	English (US)
Pages (from-to)	860-864
Number of pages	5
Journal	Plant Cell Reports
Volume	21
Issue number	9
DOIs	https://doi.org/10.1007/s00299-003-0585-x
State	Published - Jun 1 2003

All Science Journal Classification (ASJC) codes

Agronomy and Crop Science
Plant Science

Keywords

Biolistics
Hygromycin phosphotransferase gene
Transformation
Triploid bermudagrass

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title = "Transformation of triploid bermudagrass (Cynodon dactylon x C. transvaalensis cv. TifEagle) by means of biolistic bombardment",

abstract = "A transformation system for triploid bermudagrass (Cynodon dactylon x C. transvaalensis cv. TifEagle) was established with a biolistic bombardment delivery system. Embryogenic callus was induced from stolons and maintained on Murashige and Skoog's medium supplemented with 30 μM dicamba, 20 μM benzylaminopurine, and 100 mg/l myo-inositol. Using the hygromycin phosphotransferase (hpt) gene as the selectable marker gene, we obtained 75 transgenic lines from 18 petri dishes bombarded. Integration of the hpt gene into genomic DNA and transcription of hpt was confirmed by Southern and Northern blot analyses, respectively. Through suspension culture screening, we obtained homogeneously transformed plants showing stable transcription of the hpt gene.",

keywords = "Biolistics, Hygromycin phosphotransferase gene, Transformation, Triploid bermudagrass",

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AU - Zhang, G.

AU - Lu, S.

AU - Chen, T. A.

AU - Funk, C. R.

AU - Meyer, W. A.

PY - 2003/6/1

Y1 - 2003/6/1

N2 - A transformation system for triploid bermudagrass (Cynodon dactylon x C. transvaalensis cv. TifEagle) was established with a biolistic bombardment delivery system. Embryogenic callus was induced from stolons and maintained on Murashige and Skoog's medium supplemented with 30 μM dicamba, 20 μM benzylaminopurine, and 100 mg/l myo-inositol. Using the hygromycin phosphotransferase (hpt) gene as the selectable marker gene, we obtained 75 transgenic lines from 18 petri dishes bombarded. Integration of the hpt gene into genomic DNA and transcription of hpt was confirmed by Southern and Northern blot analyses, respectively. Through suspension culture screening, we obtained homogeneously transformed plants showing stable transcription of the hpt gene.

AB - A transformation system for triploid bermudagrass (Cynodon dactylon x C. transvaalensis cv. TifEagle) was established with a biolistic bombardment delivery system. Embryogenic callus was induced from stolons and maintained on Murashige and Skoog's medium supplemented with 30 μM dicamba, 20 μM benzylaminopurine, and 100 mg/l myo-inositol. Using the hygromycin phosphotransferase (hpt) gene as the selectable marker gene, we obtained 75 transgenic lines from 18 petri dishes bombarded. Integration of the hpt gene into genomic DNA and transcription of hpt was confirmed by Southern and Northern blot analyses, respectively. Through suspension culture screening, we obtained homogeneously transformed plants showing stable transcription of the hpt gene.

KW - Biolistics

KW - Hygromycin phosphotransferase gene

KW - Transformation

KW - Triploid bermudagrass

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Transformation of triploid bermudagrass (Cynodon dactylon x C. transvaalensis cv. TifEagle) by means of biolistic bombardment

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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