Transition-state responses to amino acid perturbations in yeast pyruvate decarboxylase: A carbon kinetic isotope effect study

Lan Chen, Yang Yuan, W Huskey

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Abstract

Carbon kinetic isotope effects (12C/13C at the carboxyl carbon of pyruvic acid) and their pH dependence were measured for the decarboxylation reaction catalyzed by the substrateregulated yeast pyruvate decarboxylase (PDC) and several of its site-specific variants. The active-site variants studied were E477Q, D28A and E51D; the regulatory-site variants, each with two amino acid substitutions, were C221A/C222A, C221E/C222A and C221D/ C222A. The isotope effects for the regulatory-site variants were not significantly different from the values for the wild-type enzyme (1.0046±0.0003 at pH 6.0, 25°C). For the active-site variants, the isotope effects were 1.0018±0.0009 (E477Q), 1.0398±0.0021 (D28A) and 1.0143±0.0011 (E51D) at pH 6.0 and 25°C. The results were interpreted in terms of shifts in the rate-limiting steps and uniform binding changes in the free-energy profiles for decarboxylation phase of the PDC reaction.

Original languageEnglish (US)
Pages (from-to)572-578
Number of pages7
JournalJournal of Physical Organic Chemistry
Volume17
Issue number6-7 SPEC.ISS.
Publication statusPublished - Jun 1 2004

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All Science Journal Classification (ASJC) codes

  • Physical and Theoretical Chemistry
  • Organic Chemistry

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