Translation of psbA mRNA is regulated by light via the 5'-untranslated region in tobacco plastids

Jeffrey M. Staub, Pal Maliga

Research output: Contribution to journalArticlepeer-review

135 Scopus citations

Abstract

The plastid psbA gene encodes the 32 kDa D1 polypeptide of photosystem II. It has previously been shown that the initiation of psbA mRNA translation in tobacco is regulated by sequences outside of the coding region. To identify the cis-acting regulatory sequences involved in the translational control, a series of chimeric uidA genes, encoding the β-glucuronidase (GUS) reporter enzyme, were introduced into the plastid genome. GUS accumulation in response to the light (135- to 200-fold), and the arrest of uidA mRNA translation in light-grown seedlings following transfer for 2 h to the dark, was observed only if the transgenes contained the psbA 5'-untranslated leader region (UTR). Changes in GUS accumulation were accompanied by little or no changes in the uidA mRNA levels. The data indicate that the initiation of D1 translation in tobacco plastids is controlled via the psbA 5'-UTR.

Original languageEnglish (US)
Pages (from-to)547-553
Number of pages7
JournalPlant Journal
Volume6
Issue number4
StatePublished - 1994
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science
  • Cell Biology

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