Triangulation of proteins in the 30 S ribosomal subunit of Escherichia coli

P. B. Moore, J. A. Langer, B. P. Schoenborn, D. M. Engelaman

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24 Scopus citations

Abstract

Thermal neutron radiation has been used for solution scattering experiments on 30 S ribosomal subunits in which specific proteins have been inserted in deuterated form by reconstitution. The data enable us to estimate the distances separating the centers of mass of six protein pairs S3-S4 (61 Å), S3-S5 (55 Å), S3-S8 (77 Å), S4-S7 (86 Å), S4-S8 (62 Å) and S5-S6 (96 Å). Three triangular relationships involving S3, S4, S5, S7 and S8 have been established. Transformation of the data by Patterson inversion gives, for each protein pair, the distribution of lengths of all possible vectors between the two proteins. Model calculations show that the form of the distribution is a function of the shape and orientation of the proteins. The data support the view that many of these proteins are elongated.

Original languageEnglish (US)
Pages (from-to)199-227
Number of pages29
JournalJournal of molecular biology
Volume112
Issue number2
DOIs
StatePublished - May 15 1977
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

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