Tributyltin is a potent inducer of the heat shock response in human diploid fibroblasts

Hua Zhang, Alice Y.‐C Liu

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Submicromolar concentrations of tributyltin (TBT), a commercially used organotin compound, were found to induce the expression of several stress proteins, most notably HSP89 and HSP70, in IMR‐90 human diploid fibroblats in a time‐ and dose‐dependent manner. This induction can be demonstrated by quantitation of 1) synthesis of the heat shock proteins (HSPs), 2) relative abundance of mRNA of hsp70, and 3) transient expression of a human hsp70 promoter driven reporter gene. TBT also increased the abundance of mRNA of heme oxygenase, whereas heat shock was without effect. Analysis of protein binding to a consensus heat shock element (HSE)by electrophoretic mobility shift assay suggests that the induction of the heat shock response by TBT was attributable to activation of the heat shock transcription factor (HSTF). © 1992 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)460-466
Number of pages7
JournalJournal of Cellular Physiology
Volume153
Issue number3
DOIs
StatePublished - Dec 1992

All Science Journal Classification (ASJC) codes

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

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