Trophic shift of stable isotopes and fatty acids in Collembola on bacterial diets

Dominique Haubert; Max M. Häggblom; Reinhard Langel; Stefan Scheu; Liliane Ruess

doi:10.1016/j.soilbio.2005.11.031

Trophic shift of stable isotopes and fatty acids in Collembola on bacterial diets

Dominique Haubert, Max M. Häggblom, Reinhard Langel, Stefan Scheu, Liliane Ruess

School of Environmental and Biological Sciences, Biochemistry & Microbiology

Research output: Contribution to journal › Article › peer-review

59 Scopus citations

Abstract

Fatty acid (FA) analysis is a promising tool to study trophic relationships in soil food webs. We determined FA biomarkers to trace bacterial food sources (Bacillus megaterium, Pseudomonas putida, Enterobacter aerogenes) of Collembola (Heteromurus nitidus, Protaphorura fimata, Folsomia candida). In addition, δ¹⁵N, δ¹³C, C/N ratio, body weight and NLFA/PLFA ratio (neutral lipid/phospholipid fatty acids) of Collembola were assessed. These measures indicated that P. putida ranked first, B. megaterium second and E. aerogenes third in food quality. FAs specific for bacteria were found in the NLFAs of the Collembola reflecting the respective bacterial diet. Biomarker FAs for gram-positive bacteria were methyl branched i14:0, i15:0, a15:0 and i17:0. Consumption of gram-negative bacteria was reflected by the cyclic form cy17:0 (E. aerogenes, P. putida) and by 16:1ω5 (P. putida).

Original language	English (US)
Pages (from-to)	2004-2007
Number of pages	4
Journal	Soil Biology and Biochemistry
Volume	38
Issue number	7
DOIs	https://doi.org/10.1016/j.soilbio.2005.11.031
State	Published - Jul 2006

All Science Journal Classification (ASJC) codes

Microbiology
Soil Science

Keywords

Bacteria
Biomarker
C
Collembola
Fatty acid
Food source
N
Neutral lipids

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10.1016/j.soilbio.2005.11.031

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title = "Trophic shift of stable isotopes and fatty acids in Collembola on bacterial diets",

abstract = "Fatty acid (FA) analysis is a promising tool to study trophic relationships in soil food webs. We determined FA biomarkers to trace bacterial food sources (Bacillus megaterium, Pseudomonas putida, Enterobacter aerogenes) of Collembola (Heteromurus nitidus, Protaphorura fimata, Folsomia candida). In addition, δ15N, δ13C, C/N ratio, body weight and NLFA/PLFA ratio (neutral lipid/phospholipid fatty acids) of Collembola were assessed. These measures indicated that P. putida ranked first, B. megaterium second and E. aerogenes third in food quality. FAs specific for bacteria were found in the NLFAs of the Collembola reflecting the respective bacterial diet. Biomarker FAs for gram-positive bacteria were methyl branched i14:0, i15:0, a15:0 and i17:0. Consumption of gram-negative bacteria was reflected by the cyclic form cy17:0 (E. aerogenes, P. putida) and by 16:1ω5 (P. putida).",

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author = "Dominique Haubert and H{\"a}ggblom, {Max M.} and Reinhard Langel and Stefan Scheu and Liliane Ruess",

note = "Funding Information: Financial support of D.H. by the Deutsche Forschungsgemeinschaft (DFG, Graduiertenkolleg 340, TU Darmstadt) is gratefully acknowledged. The work of L.R. was funded by a grant of the DFG, Bonn, Germany.",

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AU - Haubert, Dominique

AU - Häggblom, Max M.

AU - Langel, Reinhard

AU - Scheu, Stefan

AU - Ruess, Liliane

N1 - Funding Information: Financial support of D.H. by the Deutsche Forschungsgemeinschaft (DFG, Graduiertenkolleg 340, TU Darmstadt) is gratefully acknowledged. The work of L.R. was funded by a grant of the DFG, Bonn, Germany.

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N2 - Fatty acid (FA) analysis is a promising tool to study trophic relationships in soil food webs. We determined FA biomarkers to trace bacterial food sources (Bacillus megaterium, Pseudomonas putida, Enterobacter aerogenes) of Collembola (Heteromurus nitidus, Protaphorura fimata, Folsomia candida). In addition, δ15N, δ13C, C/N ratio, body weight and NLFA/PLFA ratio (neutral lipid/phospholipid fatty acids) of Collembola were assessed. These measures indicated that P. putida ranked first, B. megaterium second and E. aerogenes third in food quality. FAs specific for bacteria were found in the NLFAs of the Collembola reflecting the respective bacterial diet. Biomarker FAs for gram-positive bacteria were methyl branched i14:0, i15:0, a15:0 and i17:0. Consumption of gram-negative bacteria was reflected by the cyclic form cy17:0 (E. aerogenes, P. putida) and by 16:1ω5 (P. putida).

AB - Fatty acid (FA) analysis is a promising tool to study trophic relationships in soil food webs. We determined FA biomarkers to trace bacterial food sources (Bacillus megaterium, Pseudomonas putida, Enterobacter aerogenes) of Collembola (Heteromurus nitidus, Protaphorura fimata, Folsomia candida). In addition, δ15N, δ13C, C/N ratio, body weight and NLFA/PLFA ratio (neutral lipid/phospholipid fatty acids) of Collembola were assessed. These measures indicated that P. putida ranked first, B. megaterium second and E. aerogenes third in food quality. FAs specific for bacteria were found in the NLFAs of the Collembola reflecting the respective bacterial diet. Biomarker FAs for gram-positive bacteria were methyl branched i14:0, i15:0, a15:0 and i17:0. Consumption of gram-negative bacteria was reflected by the cyclic form cy17:0 (E. aerogenes, P. putida) and by 16:1ω5 (P. putida).

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KW - Fatty acid

KW - Food source

KW - N

KW - Neutral lipids

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Trophic shift of stable isotopes and fatty acids in Collembola on bacterial diets

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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