Endothelial cells regulate multiple vascular functions, such as vascular tone, permeability, remodeling, and angiogenesis. It is known for long that cytosolic Ca 2+ level ([Ca 2+] i) and membrane potential of endothelial cells are crucial factors to initiate the signal transduction cascades, leading to diverse vascular functions. Among the various kinds of endothelial ion channels that regulate ion homeostasis, transient receptor potential (TRP) channels emerge as the prime mediators for a diverse range of vascular signaling. The characteristics of TRP channels, including subunit heteromultimerization, diverse ion selectivity, and multiple modes of activation, permit their versatile functional roles in vasculatures. Substantial amount of evidence demonstrates that many TRP channels in endothelial cells participate in physiological and pathophysiological processes of vascular system. In this article, we summarize the recent findings of TRP research in endothelial cells, aiming at providing up-to-date information to the researchers in this rapidly growing field.