TRP-PLIK, a bifunctional protein with kinase and ion channel activities

Loren Runnels, L. Yue, D. E. Clapham

Research output: Contribution to journalArticlepeer-review

565 Scopus citations

Abstract

We cloned and characterized a protein kinase and ion channel, TRP-PLIK. As part of the long transient receptor potential channel subfamily implicated in control of cell division, it is a protein that is both an ion channel and a protein kinase. TRP-PLIK phosphorylated itself, displayed a wide tissue distribution, and, when expressed in CHO-K1 cells, constituted a nonselective, calcium-permeant, 105-picosiemen, steeply outwardly rectifying conductance. The zinc finger containing α-kinase domain was functional Inactivation of the kinase activity by site-directed mutagenesis and the channel's dependence on intracellular adenosine triphosphate (ATP) demonstrated that the channel's kinase activity is essential for channel function.

Original languageEnglish (US)
Pages (from-to)1043-1047
Number of pages5
JournalScience
Volume291
Issue number5506
DOIs
StatePublished - Feb 27 2001
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • General

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