TY - JOUR
T1 - TRPA1 Contributes to Cold, Mechanical, and Chemical Nociception but Is Not Essential for Hair-Cell Transduction
AU - Kwan, Kelvin Y.
AU - Allchorne, Andrew J.
AU - Vollrath, Melissa A.
AU - Christensen, Adam P.
AU - Zhang, Duan Sun
AU - Woolf, Clifford J.
AU - Corey, David P.
N1 - Funding Information:
We thank Tam Thompsen, Yiping Zhou, and Lina Du for ES cell advice and blastocyst injections; Gui-Lan Yao and Bruce Bean for help with DRG dissection; Lisa Goodrich for the pTT0 plasmid with the IRES PLAP pA cassette; Michael Deans for advice on murine E14 ES cell culture and on generation of Trpa1 mutant mice; Paul Niksch for dissecting mouse cochleae; Joe Corey for vestibular testing; and Stéphane Maison for technical advice on auditory brainstem recordings. We are also grateful for advice and comments from members of the Woolf laboratory. Supported by NIH grants NS-039518 and NS-038253 (to C.J.W.) and DC-00304 (to D.P.C.). D.P.C. is an Investigator and K.Y.K. and M.A.V. are Associates of the Howard Hughes Medical Institute.
PY - 2006/4/20
Y1 - 2006/4/20
N2 - TRPA1, a member of the transient receptor potential (TRP) family of ion channels, is expressed by dorsal root ganglion neurons and by cells of the inner ear, where it has proposed roles in sensing sound, painful cold, and irritating chemicals. To test the in vivo roles of TRPA1, we generated a mouse in which the essential exons required for proper function of the Trpa1 gene were deleted. Knockout mice display behavioral deficits in response to mustard oil, to cold (∼0° C), and to punctate mechanical stimuli. These mice have a normal startle reflex to loud noise, a normal sense of balance, a normal auditory brainstem response, and normal transduction currents in vestibular hair cells. TRPA1 is apparently not essential for hair-cell transduction but contributes to the transduction of mechanical, cold, and chemical stimuli in nociceptor sensory neurons.
AB - TRPA1, a member of the transient receptor potential (TRP) family of ion channels, is expressed by dorsal root ganglion neurons and by cells of the inner ear, where it has proposed roles in sensing sound, painful cold, and irritating chemicals. To test the in vivo roles of TRPA1, we generated a mouse in which the essential exons required for proper function of the Trpa1 gene were deleted. Knockout mice display behavioral deficits in response to mustard oil, to cold (∼0° C), and to punctate mechanical stimuli. These mice have a normal startle reflex to loud noise, a normal sense of balance, a normal auditory brainstem response, and normal transduction currents in vestibular hair cells. TRPA1 is apparently not essential for hair-cell transduction but contributes to the transduction of mechanical, cold, and chemical stimuli in nociceptor sensory neurons.
KW - HUMDISEASE
KW - MOLNEURO
KW - SIGNALING
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U2 - 10.1016/j.neuron.2006.03.042
DO - 10.1016/j.neuron.2006.03.042
M3 - Article
C2 - 16630838
AN - SCOPUS:33646061333
SN - 0896-6273
VL - 50
SP - 277
EP - 289
JO - Neuron
JF - Neuron
IS - 2
ER -