Two-dimensional vibrational correlation spectroscopy of in vitro hydroxyapatite maturation

H. Ou-Yang, E. P. Paschalis, A. L. Boskey, R. Mendelsohn

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40 Scopus citations


Two-dimensional (2-D) Raman and 2-D IR correlation spectroscopy are applied to analyze changes in the v4 region of the IR spectrum and in the v1 region of the Raman spectrum during the maturation of hydroxyapatite (HA) following the solution-mediated conversion of amorphous calcium phosphate (ACP) to HA. The v1 region of the Raman spectrum exhibits a frequency shift and sharpening during the maturation. Comparison of the experimental and simulated 2-D plots for this process suggests that the shift of a single peak, rather than a change in the relative intensity of two overlapped bands, is responsible for the observed spectral changes. The v4 mode of the PO4/3- ion (T2 symmetry in the free species) splits into a triplet with components near 563, 575, and 603 cm-1 in HA. In addition, broad features appear at 540 and 617 cm-1. During the latest stages of the maturation, an OH- librational mode develops at ~632 cm-1. Changes in the relative intensities of three components of the v4 mode are not all correlated with each other. The synchronous 2-D plots reveal that the 563 and 603 cm-1 pair are positively correlated while the feature at 575 cm-1 is absent. A 587 cm-1 mode arising from ACP is negatively correlated with the 563 and 603 cm-1 pair and is both synchronously (positively) and asynchronously correlated with the 540 cm-1 feature during the early stages of the maturation but is absent from 2-D plots of the later stages of the maturation. Cross correlations between the v4 mode and the v1,v3 contour generally confirm and extend previous assignments for the latter spectral region. Finally, the suitability of the 2-D approach for analysis of IR spectral images is examined through studies of HA crystallinity in a human iliac crest biopsy sample. Trabecular bone contains a fraction of HA that is more crystalline and mature than could be achieved in vitro during the room temperature ACP → HA interconversion. (C) 2000 John Wiley and Sons, Inc.

Original languageEnglish (US)
Pages (from-to)129-139
Number of pages11
JournalBiopolymers - Biospectroscopy Section
Issue number3
StatePublished - 2000

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Biomaterials
  • Organic Chemistry


  • 2-D correlation spectroscopy
  • Hydroxyapatite
  • IR spectroscopy
  • In vitro
  • Maturation
  • Raman spectroscopy


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