Two Monoclonal Antibodies Recognizing Carbohydrate Epitopes on Neural Adhesion Molecules Interfere with Cell Interactions

Thomas Fahrig, Brigitte Schmitz, Dieter Weber, Andrea Kücherer‐Ehret, Andreas Faissner, Melitta Schachner

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

We have studied two monoclonal antibodies raised against crude fractions of membrane glycoproteins from adult mouse brain and found them to react with two carbohydrate epitopes expressed on several neural cell adhesion molecules. Other identified and unidentified glycoproteins from different cell types, organs and species were also recognized by these antibodies. Both epitopes are N‐glycosidically linked mannosidic or hybrid type oligosaccharides and co‐expressed on all the glycoproteins so far tested. In spite of their remarkable similarities, the glycan epitopes are different as shown by ELISA competition assays. In microexplant outgrowth and cell adhesion assays, both antibodies interfere with neural cell adhesion, migration, and neurite outgrowth. These observations, together with previous studies on the L2/HNK‐1 glycan (Künemund et al., 1988), indicate that adhesion molecules carry various carbohydrate epitopes mediating different cell interactions in in vitro assays.

Original languageEnglish (US)
Pages (from-to)153-161
Number of pages9
JournalEuropean Journal of Neuroscience
Volume2
Issue number2
DOIs
StatePublished - Feb 1990
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Keywords

  • L3 epitope
  • L4 epitope
  • cell adhesion
  • neurite outgrowth

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