Two Supporting Factors Greatly Improve the Efficiency of Human iPSC Generation

Yang Zhao, Xiaolei Yin, Han Qin, Fangfang Zhu, Haisong Liu, Weifeng Yang, Qiang Zhang, Chengang Xiang, Pingping Hou, Zhihua Song, Yanxia Liu, Jun Yong, Pengbo Zhang, Jun Cai, Meng Liu, Honggang Li, Yanqin Li, Xiuxia Qu, Kai Cui, Weiqi ZhangTingting Xiang, Yetao Wu, Yiding Zhao, Chun Liu, Chen Yu, Kehu Yuan, Jinning Lou, Mingxiao Ding, Hongkui Deng

Research output: Contribution to journalLetterpeer-review

405 Scopus citations

Abstract

Human fibroblasts can be induced into pluripotent stem cells (iPSCs), but the reprogramming efficiency is quite low. Here, we screened a panel of candidate factors in the presence of OCT4, SOX2, KLF4, and c-MYC in an effort to improve the reprogramming efficiency from human adult fibroblasts. We found that p53 siRNA and UTF1 enhanced the efficiency of iPSC generation up to 100-fold, even when the oncogene c-MYC was removed from the combinations.

Original languageEnglish (US)
Pages (from-to)475-479
Number of pages5
JournalCell Stem Cell
Volume3
Issue number5
DOIs
StatePublished - Nov 6 2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Genetics
  • Cell Biology

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