Two transcriptionally active OmpR mutants that do not require phosphorylation by EnvZ in an Escherichia coli cell-free system

V. Bowrin, R. Brissette, M. Inouye

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4 Scopus citations

Abstract

D55Q-T83A and D55Q-G94S, two pseudorevertants of the D55Q mutant OmpR, an Escherichia coli transcriptional activator, were isolated previously by R. Brissette, K. Tsung, and M. Inouye (J. Bacteriol. 173:3749-3755, 1991). These pseudorevertant OmpR proteins were purified and examined for their function as transcriptional activators in a cell-free system with an ompF DNA fragment. These proteins were transcriptionally active, even after acid treatment, whereas the wild-type OmpR was completely inactive after the same treatment. Phosphorylation of acid-treated wild-type OmpR with an EnvZ11 membrane fraction and ATP restored transcriptional activity, whereas the activities of the mutant OmpR proteins did not change after phosphorylation.

Original languageEnglish (US)
Pages (from-to)6685-6687
Number of pages3
JournalJournal of bacteriology
Volume174
Issue number20
DOIs
StatePublished - 1992

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

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