Tyrosine sulfation of arylsulfatase A and its peptide

Chinnaswamy Kasinathan; Smith Jean; Paul Manowitz

doi:10.2174/092986606775974348

Tyrosine sulfation of arylsulfatase A and its peptide

Chinnaswamy Kasinathan, Smith Jean, Paul Manowitz

Research output: Contribution to journal › Article › peer-review

Abstract

Purified human liver arylsulfatase A (ASA) as well as an ASA peptide (residues 28-39) were sulfated by tyrosyl protein sulfotransferase in vitro. The media, but not the cell lysate, of normal human fibroblasts contained a tyrosine sulfated protein (pI = 4.5-5.5). This protein was not present in either media or cell lysate of human fibroblasts lacking ASA protein. These results suggest that tyrosine sulfation facilitates secretion of ASA and that this may have pathophysiological consequences.

Original language	English (US)
Pages (from-to)	357-361
Number of pages	5
Journal	Protein and Peptide Letters
Volume	13
Issue number	4
DOIs	https://doi.org/10.2174/092986606775974348
State	Published - Apr 2006

All Science Journal Classification (ASJC) codes

Structural Biology
Biochemistry

Keywords

Arylsulfatase A
Cellular regulation
Protein secretion
Tyrosine sulfation

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10.2174/092986606775974348

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title = "Tyrosine sulfation of arylsulfatase A and its peptide",

abstract = "Purified human liver arylsulfatase A (ASA) as well as an ASA peptide (residues 28-39) were sulfated by tyrosyl protein sulfotransferase in vitro. The media, but not the cell lysate, of normal human fibroblasts contained a tyrosine sulfated protein (pI = 4.5-5.5). This protein was not present in either media or cell lysate of human fibroblasts lacking ASA protein. These results suggest that tyrosine sulfation facilitates secretion of ASA and that this may have pathophysiological consequences.",

keywords = "Arylsulfatase A, Cellular regulation, Protein secretion, Tyrosine sulfation",

author = "Chinnaswamy Kasinathan and Smith Jean and Paul Manowitz",

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T1 - Tyrosine sulfation of arylsulfatase A and its peptide

AU - Kasinathan, Chinnaswamy

AU - Jean, Smith

AU - Manowitz, Paul

PY - 2006/4

Y1 - 2006/4

N2 - Purified human liver arylsulfatase A (ASA) as well as an ASA peptide (residues 28-39) were sulfated by tyrosyl protein sulfotransferase in vitro. The media, but not the cell lysate, of normal human fibroblasts contained a tyrosine sulfated protein (pI = 4.5-5.5). This protein was not present in either media or cell lysate of human fibroblasts lacking ASA protein. These results suggest that tyrosine sulfation facilitates secretion of ASA and that this may have pathophysiological consequences.

AB - Purified human liver arylsulfatase A (ASA) as well as an ASA peptide (residues 28-39) were sulfated by tyrosyl protein sulfotransferase in vitro. The media, but not the cell lysate, of normal human fibroblasts contained a tyrosine sulfated protein (pI = 4.5-5.5). This protein was not present in either media or cell lysate of human fibroblasts lacking ASA protein. These results suggest that tyrosine sulfation facilitates secretion of ASA and that this may have pathophysiological consequences.

KW - Arylsulfatase A

KW - Cellular regulation

KW - Protein secretion

KW - Tyrosine sulfation

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Tyrosine sulfation of arylsulfatase A and its peptide

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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