TY - JOUR
T1 - Tyrosylprotein Sulfotransferase Activity is Increased in Human Gastric Mucosa of Alcoholics
AU - Sundaram, P.
AU - Slomiany, B. L.
AU - Slomiany, A.
AU - Rajiyah, G.
AU - Khan, M. Y.
AU - Kasinathan, C.
PY - 1992/10
Y1 - 1992/10
N2 - Sulfation plays a major role in the processing of secretory proteins. We report here on tyrosylprotein sulfotransferase activity in human gastric mucosa of normal and alcoholics. The tyrosylprotein snlfotransferase was identified in the Golgi‐enriched fraction. In alcoholics, the activity of sulfotransferase was 2‐ to 3‐ fold higher than in normals. However, no change in the activity of UDP‐glucose‐ceramide glucosyltransferase, a marker for Golgi, between alcoholics and normals was observed. The tyrosylprotein sulfotransferase enzyme required Triton X‐100, MnCl2 and 5′‐AMP, and obtained optimum activity at pH 6.8 in the presence of 0.5% Triton X‐100, 20 mM MnCl2 50 mM NaF, and 2 mM 5′‐AMP. The apparent Km for poly‐Glu6, Ala3, Tyr1 (EAY; 47,000) was 1.9 × 10‐6 M and for 3′‐phosphoadenosine 5′‐phosphosulfate (PAPS), 1.4 × 10‐6 M. The results suggest that alcohol abuse causes enhancement in the expression of gastric mucosal tyrosylprotein sulfotransferase activity.
AB - Sulfation plays a major role in the processing of secretory proteins. We report here on tyrosylprotein sulfotransferase activity in human gastric mucosa of normal and alcoholics. The tyrosylprotein snlfotransferase was identified in the Golgi‐enriched fraction. In alcoholics, the activity of sulfotransferase was 2‐ to 3‐ fold higher than in normals. However, no change in the activity of UDP‐glucose‐ceramide glucosyltransferase, a marker for Golgi, between alcoholics and normals was observed. The tyrosylprotein sulfotransferase enzyme required Triton X‐100, MnCl2 and 5′‐AMP, and obtained optimum activity at pH 6.8 in the presence of 0.5% Triton X‐100, 20 mM MnCl2 50 mM NaF, and 2 mM 5′‐AMP. The apparent Km for poly‐Glu6, Ala3, Tyr1 (EAY; 47,000) was 1.9 × 10‐6 M and for 3′‐phosphoadenosine 5′‐phosphosulfate (PAPS), 1.4 × 10‐6 M. The results suggest that alcohol abuse causes enhancement in the expression of gastric mucosal tyrosylprotein sulfotransferase activity.
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U2 - 10.1111/j.1572-0241.1992.tb02696.x
DO - 10.1111/j.1572-0241.1992.tb02696.x
M3 - Article
C2 - 1415104
AN - SCOPUS:0026801339
VL - 87
SP - 1460
EP - 1463
JO - American Journal of Gastroenterology
JF - American Journal of Gastroenterology
SN - 0002-9270
IS - 10
ER -