TY - JOUR
T1 - U1 snRNP inhibits pre-mRNA polyadenylation through a direct interaction between U1 70K and poly(A) polymerase
AU - Gunderson, Samuel I.
AU - Polycarpou-Schwarz, Maria
AU - Mattaj, Iain W.
N1 - Funding Information:
We thank Stephan Vagner, Bertrand Seraphin, Juan Valcarcel, Silvie Beaudenon, Jon Huibregtse, Megerditch Kiledjian, Isabel Palacios, and Gert-Jan Arts for critical reading of the manuscript; Silke Bienroth and Walter Keller for purified CPSF; and Elmar Wahle for bovine PAP purified from calf thymus. The work was supported by the European Molecular Biology Laboratory and the Deutsche Forschungsgemeinschaft Schwerpunktprogramm “RNA Biochimie.”
PY - 1998/1
Y1 - 1998/1
N2 - It has previously been shown in vivo that bovine papillomavirus represses its late gene expression via a 5′ splice site sequence located upstream of the late polyadenylation signal. Here, the mechanism of repression is determined by in vitro analysis. U1 snRNP binding to the 5′ splice site results in inhibition of polyadenylation via a direct interaction with poly(A) polymerase (PAP). Although the inhibitory mechanism is similar to that used in U1A autoregulation, U1A within the U1 snRNP does not contribute to PAP inhibition. Instead the U1 70K protein, when bound to U1 snRNA, both interacts with and inhibits PAP. Conservation of the U1 70K inhibitory domains suggests that polyadenylation regulation via PAP inhibition may be more widespread than previously thought.
AB - It has previously been shown in vivo that bovine papillomavirus represses its late gene expression via a 5′ splice site sequence located upstream of the late polyadenylation signal. Here, the mechanism of repression is determined by in vitro analysis. U1 snRNP binding to the 5′ splice site results in inhibition of polyadenylation via a direct interaction with poly(A) polymerase (PAP). Although the inhibitory mechanism is similar to that used in U1A autoregulation, U1A within the U1 snRNP does not contribute to PAP inhibition. Instead the U1 70K protein, when bound to U1 snRNA, both interacts with and inhibits PAP. Conservation of the U1 70K inhibitory domains suggests that polyadenylation regulation via PAP inhibition may be more widespread than previously thought.
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U2 - 10.1016/S1097-2765(00)80026-X
DO - 10.1016/S1097-2765(00)80026-X
M3 - Article
C2 - 9659922
AN - SCOPUS:0031610367
SN - 1097-2765
VL - 1
SP - 255
EP - 264
JO - Molecular cell
JF - Molecular cell
IS - 2
ER -