Ultra-structural identification of interstitial cells of Cajal in the zebrafish Danio rerio

Evan R. Ball, Miho Matsuda, Louis Dye, Victoria Hoffmann, Patricia M. Zerfas, Eva Szarek, Adam Rich, Ajay B. Chitnis, Constantine A. Stratakis

Research output: Contribution to journalArticle

12 Scopus citations


The interstitial cells of Cajal (ICCs) are important mediators of gastrointestinal (GI) motility because of their role as pacemakers in the GI tract. In addition to their function, ICCs are also structurally distinct cells most easily identified by their ultra-structural features and expression of the tyrosine kinase receptor c-KIT. ICCs have been described inmammals, rodents, birds, reptiles, and amphibians, but there are no reports at the ultra-structural level of ICCs within the GI tract of an organism from the teleost lineage. We describe the presence of cells in the muscularis of the zebrafish intestine; these cells have similar features to ICCs in other vertebrates. The ICC-like cells are associated with the muscularis, are more electron-dense than surrounding smooth muscle cells, possess long cytoplasmic processes and mitochondria, and are situated opposing enteric nervous structures. In addition, immunofluorescent and immunoelectron-microscopic studies with antibodies targeting the zebrafish ortholog of a putative ICC marker, c-KIT (kita), showed c-kit immunoreactivity in zebrafish ICCs. Taken together, these data represent the first ultra-structural characterization of cells in the muscularis of the zebrafish Danio rerio and suggest that ICC differentiation in vertebrate evolution dates back to the teleost lineage.

Original languageEnglish (US)
Pages (from-to)483-491
Number of pages9
JournalCell And Tissue Research
Issue number2
Publication statusPublished - May 24 2012
Externally publishedYes


All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Cell Biology
  • Histology

Cite this

Ball, E. R., Matsuda, M., Dye, L., Hoffmann, V., Zerfas, P. M., Szarek, E., ... Stratakis, C. A. (2012). Ultra-structural identification of interstitial cells of Cajal in the zebrafish Danio rerio. Cell And Tissue Research, 349(2), 483-491. https://doi.org/10.1007/s00441-012-1434-4