Unique evolution characteristics of the envelope protein of EIAV LN40, a virulent strain of equine infectious anemia virus

Xuefeng Wang, Shuai Wang, Yuezhi Lin, Chenggang Jiang, Jian Ma, Liping Zhao, Xiaoling Lv, Fenglong Wang, Rongxian Shen, Jianhua Zhou

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


The Chinese equine infectious anemia virus (EIAV) virulent strain EIAV LN40 is derived from a naturally occurring virus by continuously passing in horses for 16 generations. Its genome sequence is 23% different from that of the American strains or the Japanese strains, and the variation of envelope gp90 surface unit (SU) is as high as 41%. In this study, evolutions of the EIAVLN40 gp90 gene in four infected horses were analyzed. Results showed that new quasispecies arose in the early stage of infection in all EIAVLN40-infected horses. These quasispecies belonged to branches different from EIAVLN40 in a phylogenetic tree. In contrast, the gp90 sequences of viruses isolated after disease onset remained in the same phylogenetic branch as EIAVLN40, with some having exactly the same sequences. The glycosylation sites 191NSSN and 237NNTW in the V3 and V4 region present or absent simultaneously in most of the predicted amino acid sequences. Changes in the glycosylation sites within V3, V4, and V5 regions are usually associated with the disease status. Glycosylation sites (191NSSN, 237NNTW, and 280NDTS) within these three regions were present in EIAVLN40 and most of the quasispecies isolated after, but not before disease onset. These unique evolutionary characteristics of SU have not been reported for EIAV and other lentiviruses. Our results provide a reference for a further understanding of the mechanism underlying the persistent infection and escape from immune surveillance of EIAV.

Original languageEnglish (US)
Pages (from-to)220-228
Number of pages9
JournalVirus Genes
Issue number2
StatePublished - Apr 2011
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Virology


  • Envelope protein
  • Equine infectious anemia virus
  • Evolution characteristics


Dive into the research topics of 'Unique evolution characteristics of the envelope protein of EIAV LN40, a virulent strain of equine infectious anemia virus'. Together they form a unique fingerprint.

Cite this