Unique sequence arrangement of ribosomal genes in the palindromic rDNA molecule of physarum polycephalum

Gerald R. Campbell; Virginia C. Littau; Peter W. Melera; Vincent G. Allfrey; Edward M. Johnson

doi:10.1093/nar/6.4.1433

Unique sequence arrangement of ribosomal genes in the palindromic rDNA molecule of physarum polycephalum

Gerald R. Campbell, Virginia C. Littau, Peter W. Melera, Vincent G. Allfrey, Edward M. Johnson

Research output: Contribution to journal › Article › peer-review

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Abstract

R-loop and restriction mapping procedures reveal the organization of coding regions at each end of the giant rDNA palindrome of Physarum polycephalum. A 19S coding region of 2.10±0.21 kb is located at each end of a very long central spacer (35.64±2.08 kb). An Internal spacer of 1.66±0.12 kb lies distal to the 19S gene. The 5.8S rRNA coding region 1s located in this spacer. The 26S gene lies distal to the internal spacer. The 26S gene is unusual among those of eukaryotes in that it consists of 3 coding regions (α, β and γ) interrupted by 2 intervening sequences. The 26S α (most central) coding segment of 2.41±0.33 kb 1s separated from the 26S β segment by an intervening sequence of 0.68±0.13 kb. The 26S β segment (0.70±0.11 kb) is separated from the most distal 26S γ segment (0.59±0.14 kb) by an intervening sequence of 1.21±0.14 kb. The 2 intervening sequences are present in at least 88% of ribosomal genes from active plasmodia, Indicating that genes containing these sequences are transcribed. The rDNA termini contain a heterogeneous region which varies in length by±300 base pairs.

Original language	English (US)
Pages (from-to)	1433-1447
Number of pages	15
Journal	Nucleic acids research
Volume	6
Issue number	4
DOIs	https://doi.org/10.1093/nar/6.4.1433
State	Published - Apr 1979
Externally published	Yes

All Science Journal Classification (ASJC) codes

Genetics

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10.1093/nar/6.4.1433

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@article{e7a717760fe1422ab58462f659a5a8da,

title = "Unique sequence arrangement of ribosomal genes in the palindromic rDNA molecule of physarum polycephalum",

abstract = "R-loop and restriction mapping procedures reveal the organization of coding regions at each end of the giant rDNA palindrome of Physarum polycephalum. A 19S coding region of 2.10±0.21 kb is located at each end of a very long central spacer (35.64±2.08 kb). An Internal spacer of 1.66±0.12 kb lies distal to the 19S gene. The 5.8S rRNA coding region 1s located in this spacer. The 26S gene lies distal to the internal spacer. The 26S gene is unusual among those of eukaryotes in that it consists of 3 coding regions (α, β and γ) interrupted by 2 intervening sequences. The 26S α (most central) coding segment of 2.41±0.33 kb 1s separated from the 26S β segment by an intervening sequence of 0.68±0.13 kb. The 26S β segment (0.70±0.11 kb) is separated from the most distal 26S γ segment (0.59±0.14 kb) by an intervening sequence of 1.21±0.14 kb. The 2 intervening sequences are present in at least 88% of ribosomal genes from active plasmodia, Indicating that genes containing these sequences are transcribed. The rDNA termini contain a heterogeneous region which varies in length by±300 base pairs.",

author = "Campbell, {Gerald R.} and Littau, {Virginia C.} and Melera, {Peter W.} and Allfrey, {Vincent G.} and Johnson, {Edward M.}",

note = "Funding Information: ACKNOWLEDGEMENTS We thank Dr. M.T. Hsu, of The Rockefeller University, for valuable experimental advice concerning R-loop mapping. Ms. W.Steer and Drs. H.R. Matthews and E.M. Bradbury, of Portsmouth Polytechnic, UK, provided unpublished observations concerning mapping of restriction fragments. Work was supported by the NSF (PCM76 19926) ,.the American Cancer Society (NP 228H) and the NIH (GM17383).",

year = "1979",

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doi = "10.1093/nar/6.4.1433",

language = "English (US)",

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T1 - Unique sequence arrangement of ribosomal genes in the palindromic rDNA molecule of physarum polycephalum

AU - Campbell, Gerald R.

AU - Littau, Virginia C.

AU - Melera, Peter W.

AU - Allfrey, Vincent G.

AU - Johnson, Edward M.

N1 - Funding Information: ACKNOWLEDGEMENTS We thank Dr. M.T. Hsu, of The Rockefeller University, for valuable experimental advice concerning R-loop mapping. Ms. W.Steer and Drs. H.R. Matthews and E.M. Bradbury, of Portsmouth Polytechnic, UK, provided unpublished observations concerning mapping of restriction fragments. Work was supported by the NSF (PCM76 19926) ,.the American Cancer Society (NP 228H) and the NIH (GM17383).

PY - 1979/4

Y1 - 1979/4

N2 - R-loop and restriction mapping procedures reveal the organization of coding regions at each end of the giant rDNA palindrome of Physarum polycephalum. A 19S coding region of 2.10±0.21 kb is located at each end of a very long central spacer (35.64±2.08 kb). An Internal spacer of 1.66±0.12 kb lies distal to the 19S gene. The 5.8S rRNA coding region 1s located in this spacer. The 26S gene lies distal to the internal spacer. The 26S gene is unusual among those of eukaryotes in that it consists of 3 coding regions (α, β and γ) interrupted by 2 intervening sequences. The 26S α (most central) coding segment of 2.41±0.33 kb 1s separated from the 26S β segment by an intervening sequence of 0.68±0.13 kb. The 26S β segment (0.70±0.11 kb) is separated from the most distal 26S γ segment (0.59±0.14 kb) by an intervening sequence of 1.21±0.14 kb. The 2 intervening sequences are present in at least 88% of ribosomal genes from active plasmodia, Indicating that genes containing these sequences are transcribed. The rDNA termini contain a heterogeneous region which varies in length by±300 base pairs.

AB - R-loop and restriction mapping procedures reveal the organization of coding regions at each end of the giant rDNA palindrome of Physarum polycephalum. A 19S coding region of 2.10±0.21 kb is located at each end of a very long central spacer (35.64±2.08 kb). An Internal spacer of 1.66±0.12 kb lies distal to the 19S gene. The 5.8S rRNA coding region 1s located in this spacer. The 26S gene lies distal to the internal spacer. The 26S gene is unusual among those of eukaryotes in that it consists of 3 coding regions (α, β and γ) interrupted by 2 intervening sequences. The 26S α (most central) coding segment of 2.41±0.33 kb 1s separated from the 26S β segment by an intervening sequence of 0.68±0.13 kb. The 26S β segment (0.70±0.11 kb) is separated from the most distal 26S γ segment (0.59±0.14 kb) by an intervening sequence of 1.21±0.14 kb. The 2 intervening sequences are present in at least 88% of ribosomal genes from active plasmodia, Indicating that genes containing these sequences are transcribed. The rDNA termini contain a heterogeneous region which varies in length by±300 base pairs.

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JO - Nucleic acids research

JF - Nucleic acids research

IS - 4

ER -

Unique sequence arrangement of ribosomal genes in the palindromic rDNA molecule of physarum polycephalum

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