Up-regulation of NAD(P)H quinone oxidoreductase 1 during human liver injury

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Abstract

Aim: To investigate the expression and activity of NAD(P)H quinone oxidoreductase 1 (NQO1) in human liver specimens obtained from patients with liver damage due to acetaminophen (APAP) overdose or primary biliary cirrhosis (PBC). Methods: NQO1 activity was determined in cytos ol from normal, APAP and PBC liver specimens. Western blot and immunohistochemical staining were used to determine patterns of NQO1 expression using a specific antibody against NQO1. Results: NQO1 protein was very low in normal human livers . In both APAP and PBC livers, there was strong induction of NQO1 protein levels on Western blot. Correspondingly, significant up-regulation of enzyme activity (16- and 22-fold, P<0.05) was also observed in APAP and PBC livers, respectively. Immunohistochemical analysis highlighted injury-specific patterns of NQO1 staining in both APAP and PBC livers. Conclusion: These data demonstrate that NQO1 protein and activity are markedly induced in human livers during both APAP overdose and PBC. Up-regulation of this cytoprotective enzyme may represent an adaptive stress response to limit further disease progression by detoxifying reactive species.

Original languageEnglish (US)
Pages (from-to)1937-1940
Number of pages4
JournalWorld Journal of Gastroenterology
Volume12
Issue number12
DOIs
StatePublished - Mar 28 2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Gastroenterology

Keywords

  • APAP
  • Acetaminophen
  • Diaphorase
  • NQ01
  • Nrf2
  • PBC
  • Primary biliary cirrhosis
  • Quinone oxidoreductase

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