Up-regulation of narX, encoding a putative 'fused nitrate reductase' in anaerobic dormant Mycobacterium bovis BCG

Bernd Hutter, Thomas Dick

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

Mycobacterium tuberculosis and its closely related but non-pathogenic relative M. bovis Bacille Calmette-Guerin (BCG) have the capability to adapt to anaerobiosis by shifting down from aerobic growth to a state of non-replicating persistence or dormancy. Here, we report the results of a comparative Northern analysis of 23 genes identified in the tubercle bacillus genome project that might play a role in the energy metabolism under anaerobic conditions. The expression of a majority of the genes was found to be down-regulated in the dormant BCG culture. However, the mRNA level for narX, a putative 'fused nitrate reductase' not found in other bacteria, was strongly up-regulated in anaerobic dormant bacilli. narX is the first transcriptionally induced gene in anaerobic dormant mycobacteria and might be a useful marker for monitoring the dormancy response in infected animals. Copyright (C) 1999.

Original languageEnglish (US)
Pages (from-to)63-69
Number of pages7
JournalFEMS Microbiology Letters
Volume178
Issue number1
DOIs
StatePublished - Sep 1 1999
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

Keywords

  • Anaerobiosis
  • Dormancy
  • Gene expression
  • Nitrate reductase

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