Use of ω-aminohexyl-sepharose in the fractionation of escherichia coli B aminoacyl-tRNA synthetases

Hieronim Jakubowski

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The usefulness of aminohexyl-Sepharose in purification of E. coli B amino-acyl-tRNA synthetases is presented. The purification factors for 14 synthetases lie in the range 3- to 94-fold and the recoveries of the enzymatic activity were 30-80%, depending on the enzyme.

Original languageEnglish (US)
Pages (from-to)331-336
Number of pages6
JournalJournal of Chromatography A
Volume139
Issue number2
DOIs
StatePublished - Sep 21 1977
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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