The usefulness of aminohexyl-Sepharose in purification of E. coli B amino-acyl-tRNA synthetases is presented. The purification factors for 14 synthetases lie in the range 3- to 94-fold and the recoveries of the enzymatic activity were 30-80%, depending on the enzyme.
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Organic Chemistry