Use of negative dielectrophoresis for selective elution of protein-bound particles

Mehdi Javanmard, Sam Emaminejad, Robert W. Dutton, Ronald W. Davis

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

In this paper with the aid of negative dielectrophoresis force in conjunction with shear force and at an optimal sodium hydroxide concentration we demonstrated a switchlike functionality to elute specifically bound beads from the surface. At an optimal flow rate and sodium hydroxide concentration, negative dielectrophoresis turned on results in bead detachment, whereas when negative dielectrophoresis is off, the beads remain attached. This platform offers the potential for performing a bead-based multiplexed assay where in a single channel various regions are immobilized with a different antibody, each targeting a different antigen. To develop the proof of concept and to demonstrate the switchlike functionality in eluting specifically bound beads from the surface we looked at two different protein interactions. We chose interactions that were in the same order of magnitude in strength as typical antibody-antigen interactions. The first was protein G-IgG interaction, and the second was the interaction between anti-IgG and IgG.

Original languageEnglish (US)
Pages (from-to)1432-1438
Number of pages7
JournalAnalytical Chemistry
Volume84
Issue number3
DOIs
StatePublished - Feb 7 2012
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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