Use of phlorizin binding to demonstrate induction of intestinal glucose transporters

Ronaldo P. Ferraris, Jared M. Diamond

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

We used specific binding of phlorizin to the intact intestinal mucosa in order to measure glucose transport site density in intestines of mice fed a high-carbohydrate or no-carbohydrate diet. Nonspecific binding varied with intestinal position but showed only modest dependence on diet. Specific binding to glucose transporters was 1.9 times greater in jejunum of high-carbohydrate mice than of no-carbohydrate mice; this ratio was the same as the ratio for Vmax values of active d-glucose uptake between the two diet groups. The gradient in specific binding of phlorizin along the intestine paralleled the gradient in Vmax of glucose transport. These results directly demonstrate that the increase in intestinal glucose transport caused by a high-carbohydrate diet is due to induction of glucose transporter. They also indicate that the normal positional graident in glucose transport along the intestine arises from a gradient in transporters, induced by the normal gradient in luminal glucose concentration.

Original languageEnglish (US)
Pages (from-to)77-82
Number of pages6
JournalThe Journal of Membrane Biology
Volume94
Issue number1
DOIs
StatePublished - Feb 1 1986
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Physiology
  • Cell Biology

Keywords

  • dietaryaegulation
  • glucose transport
  • induction
  • phlorizin binding
  • small intestine

Fingerprint Dive into the research topics of 'Use of phlorizin binding to demonstrate induction of intestinal glucose transporters'. Together they form a unique fingerprint.

  • Cite this