Validated reversed phase LC method for quantitative analysis of polymethoxyflavones in citrus peel extracts

Zhenyu Wang, Shiming Li, Stephen Ferguson, Robert Goodnow, Chi Tang Ho

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


Polymethoxyflavones (PMFs), which exist exclusively in the citrus genus, have biological activities including anti-inflammatory, anticarcinogenic, and antiatherogenic properties. A validated RPLC method was developed for quantitative analysis of six major PMFs, namely nobiletin, tangeretin, sinensetin, 5,6,7,4′-tetramethoxy-flavone, 3,5,6,7,3′,4′-hexamethoxyflavone, and 3,5,6,7,8,3′,4′-heptamethoxyflavone. The polar embedded LC stationary phase was able to fully resolve the six analogues. The developed method was fully validated in terms of linearity, accuracy, precision, sensitivity, and system suitability. The LOD of the method was calculated as 0.15 μg/mL and the recovery rate was between 97.0 and 105.1%. This analytical method was successfully applied to quantify the individual PMFs in four commercially available citrus peel extracts (CPEs). Each extract shows significant difference in the PMF composition and concentration. This method may provide a simple, rapid, and reliable tool to help reveal the correlation between the bioactivity of the PMF extracts and the individual PMF content.

Original languageEnglish (US)
Pages (from-to)30-37
Number of pages8
JournalJournal of Separation Science
Issue number1
StatePublished - Jan 2008

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Filtration and Separation


  • Citrus peel extract
  • Liquid chromatography
  • Method validation
  • Polymethoxyflavones
  • Quantitative analysis

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