Varunaivibrio sulfuroxidans gen. Nov., sp. nov., a facultatively chemolithoautotrophic, mesophilic alphaproteobacterium from a shallow-water gas vent at Tor Caldara, Tyrrhenian Sea

Sushmita Patwardhan, Costantino Vetriani

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4 Scopus citations

Abstract

A mesophilic, facultatively anaerobic, facultatively chemolithoautotrophic bacterium, designated strain TC8T, was isolated from a sulfidic shallow-water marine gas vent located at Tor Caldara, in the Tyrrhenian Sea, Italy. Cells were Gram-stain-negative curved rods with one or more polar flagella. Cells were approximately 1–1.5 μm in length and 0.6 μm in width. Strain TC8T grew between 20 and 35 °C (optimum 30 °C), with between 5 and 45 g NaCl l-1 (optimum 15–20 g l-1) and between pH 4.5 and 8.5 (optimum pH 6.0–7.0). The generation time under optimal conditions was 8 h. Strain TC8T was a facultative chemolithoautotroph also capable of using organic substrates as electron donors and carbon sources. Chemolithoautotrophic growth occurred with sulfur and thiosulfate as the electron donors, CO2 as the carbon source, and nitrate, oxygen (5 %, v/v) and ferric iron as the electron acceptors. Chemoorganoheterotrophic growth occurred with tryptone, peptone, Casamino acids, pyruvate and glycerol as substrates, while chemolithoherotrophic growth occurred with D(+)-glucose, sucrose, yeast extract, acetate, lactate, citrate and L-glutamine. The G+C content of the genomic DNA was 59.9 mol%. Phylogenetic analysis of the 16S rRNA gene sequence of strain TC8T showed that this organism formed a lineage within the family Rhodospirillaceae, which branched separately from the two closest relatives, Magnetovibrio blakemorei MV1T (91.25% similarity) and Magnetospira thiophila MMS-1T (90.13 %). Based on phylogenetic, physiological and chemotaxonomic characteristics, it is proposed that the organism represents a novel species of a new genus within the family Rhodospirillaceae, Varunaivibrio sulfuroxidans gen. nov., sp. nov. The type strain of Varunaivibrio sulfuroxidans is TC8T (=DSM 101688T=JCM 31027T).

Original languageEnglish (US)
Article number001235
Pages (from-to)3579-3584
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume66
Issue number9
DOIs
StatePublished - Sep 2016

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Keywords

  • Alphaproteobacteria
  • Chemosynthesis
  • Gas vents
  • Mediterranean Sea
  • Mesophiles
  • Nitrate
  • Oxygen and iron respiration
  • Sulfur-oxidation
  • Tor Caldara

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