Varunaivibrio sulfuroxidans gen. Nov., sp. nov., a facultatively chemolithoautotrophic, mesophilic alphaproteobacterium from a shallow-water gas vent at Tor Caldara, Tyrrhenian Sea

Sushmita Patwardhan, Costantino Vetriani

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3 Citations (Scopus)

Abstract

A mesophilic, facultatively anaerobic, facultatively chemolithoautotrophic bacterium, designated strain TC8T, was isolated from a sulfidic shallow-water marine gas vent located at Tor Caldara, in the Tyrrhenian Sea, Italy. Cells were Gram-stain-negative curved rods with one or more polar flagella. Cells were approximately 1–1.5 μm in length and 0.6 μm in width. Strain TC8T grew between 20 and 35 °C (optimum 30 °C), with between 5 and 45 g NaCl l-1 (optimum 15–20 g l-1) and between pH 4.5 and 8.5 (optimum pH 6.0–7.0). The generation time under optimal conditions was 8 h. Strain TC8T was a facultative chemolithoautotroph also capable of using organic substrates as electron donors and carbon sources. Chemolithoautotrophic growth occurred with sulfur and thiosulfate as the electron donors, CO2 as the carbon source, and nitrate, oxygen (5 %, v/v) and ferric iron as the electron acceptors. Chemoorganoheterotrophic growth occurred with tryptone, peptone, Casamino acids, pyruvate and glycerol as substrates, while chemolithoherotrophic growth occurred with D(+)-glucose, sucrose, yeast extract, acetate, lactate, citrate and L-glutamine. The G+C content of the genomic DNA was 59.9 mol%. Phylogenetic analysis of the 16S rRNA gene sequence of strain TC8T showed that this organism formed a lineage within the family Rhodospirillaceae, which branched separately from the two closest relatives, Magnetovibrio blakemorei MV1T (91.25% similarity) and Magnetospira thiophila MMS-1T (90.13 %). Based on phylogenetic, physiological and chemotaxonomic characteristics, it is proposed that the organism represents a novel species of a new genus within the family Rhodospirillaceae, Varunaivibrio sulfuroxidans gen. nov., sp. nov. The type strain of Varunaivibrio sulfuroxidans is TC8T (=DSM 101688T=JCM 31027T).

Original languageEnglish (US)
Article number001235
Pages (from-to)3579-3584
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume66
Issue number9
DOIs
StatePublished - Sep 2016

Fingerprint

Alphaproteobacteria
Tyrrhenian Sea
alpha-Proteobacteria
Rhodospirillaceae
Oceans and Seas
shallow water
Gases
gases
Electrons
electron
Water
new species
Chemoautotrophic Growth
Carbon
gas
phylogenetics
electrons
substrate
Thiosulfates
Peptones

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Keywords

  • Alphaproteobacteria
  • Chemosynthesis
  • Gas vents
  • Mediterranean Sea
  • Mesophiles
  • Nitrate
  • Oxygen and iron respiration
  • Sulfur-oxidation
  • Tor Caldara

Cite this

@article{3c8eadfe6784429f983fecaad9c7ff94,
title = "Varunaivibrio sulfuroxidans gen. Nov., sp. nov., a facultatively chemolithoautotrophic, mesophilic alphaproteobacterium from a shallow-water gas vent at Tor Caldara, Tyrrhenian Sea",
abstract = "A mesophilic, facultatively anaerobic, facultatively chemolithoautotrophic bacterium, designated strain TC8T, was isolated from a sulfidic shallow-water marine gas vent located at Tor Caldara, in the Tyrrhenian Sea, Italy. Cells were Gram-stain-negative curved rods with one or more polar flagella. Cells were approximately 1–1.5 μm in length and 0.6 μm in width. Strain TC8T grew between 20 and 35 °C (optimum 30 °C), with between 5 and 45 g NaCl l-1 (optimum 15–20 g l-1) and between pH 4.5 and 8.5 (optimum pH 6.0–7.0). The generation time under optimal conditions was 8 h. Strain TC8T was a facultative chemolithoautotroph also capable of using organic substrates as electron donors and carbon sources. Chemolithoautotrophic growth occurred with sulfur and thiosulfate as the electron donors, CO2 as the carbon source, and nitrate, oxygen (5 {\%}, v/v) and ferric iron as the electron acceptors. Chemoorganoheterotrophic growth occurred with tryptone, peptone, Casamino acids, pyruvate and glycerol as substrates, while chemolithoherotrophic growth occurred with D(+)-glucose, sucrose, yeast extract, acetate, lactate, citrate and L-glutamine. The G+C content of the genomic DNA was 59.9 mol{\%}. Phylogenetic analysis of the 16S rRNA gene sequence of strain TC8T showed that this organism formed a lineage within the family Rhodospirillaceae, which branched separately from the two closest relatives, Magnetovibrio blakemorei MV1T (91.25{\%} similarity) and Magnetospira thiophila MMS-1T (90.13 {\%}). Based on phylogenetic, physiological and chemotaxonomic characteristics, it is proposed that the organism represents a novel species of a new genus within the family Rhodospirillaceae, Varunaivibrio sulfuroxidans gen. nov., sp. nov. The type strain of Varunaivibrio sulfuroxidans is TC8T (=DSM 101688T=JCM 31027T).",
keywords = "Alphaproteobacteria, Chemosynthesis, Gas vents, Mediterranean Sea, Mesophiles, Nitrate, Oxygen and iron respiration, Sulfur-oxidation, Tor Caldara",
author = "Sushmita Patwardhan and Costantino Vetriani",
year = "2016",
month = "9",
doi = "10.1099/ijsem.0.001235",
language = "English (US)",
volume = "66",
pages = "3579--3584",
journal = "International Journal of Systematic and Evolutionary Microbiology",
issn = "1466-5026",
publisher = "Society for General Microbiology",
number = "9",

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TY - JOUR

T1 - Varunaivibrio sulfuroxidans gen. Nov., sp. nov., a facultatively chemolithoautotrophic, mesophilic alphaproteobacterium from a shallow-water gas vent at Tor Caldara, Tyrrhenian Sea

AU - Patwardhan, Sushmita

AU - Vetriani, Costantino

PY - 2016/9

Y1 - 2016/9

N2 - A mesophilic, facultatively anaerobic, facultatively chemolithoautotrophic bacterium, designated strain TC8T, was isolated from a sulfidic shallow-water marine gas vent located at Tor Caldara, in the Tyrrhenian Sea, Italy. Cells were Gram-stain-negative curved rods with one or more polar flagella. Cells were approximately 1–1.5 μm in length and 0.6 μm in width. Strain TC8T grew between 20 and 35 °C (optimum 30 °C), with between 5 and 45 g NaCl l-1 (optimum 15–20 g l-1) and between pH 4.5 and 8.5 (optimum pH 6.0–7.0). The generation time under optimal conditions was 8 h. Strain TC8T was a facultative chemolithoautotroph also capable of using organic substrates as electron donors and carbon sources. Chemolithoautotrophic growth occurred with sulfur and thiosulfate as the electron donors, CO2 as the carbon source, and nitrate, oxygen (5 %, v/v) and ferric iron as the electron acceptors. Chemoorganoheterotrophic growth occurred with tryptone, peptone, Casamino acids, pyruvate and glycerol as substrates, while chemolithoherotrophic growth occurred with D(+)-glucose, sucrose, yeast extract, acetate, lactate, citrate and L-glutamine. The G+C content of the genomic DNA was 59.9 mol%. Phylogenetic analysis of the 16S rRNA gene sequence of strain TC8T showed that this organism formed a lineage within the family Rhodospirillaceae, which branched separately from the two closest relatives, Magnetovibrio blakemorei MV1T (91.25% similarity) and Magnetospira thiophila MMS-1T (90.13 %). Based on phylogenetic, physiological and chemotaxonomic characteristics, it is proposed that the organism represents a novel species of a new genus within the family Rhodospirillaceae, Varunaivibrio sulfuroxidans gen. nov., sp. nov. The type strain of Varunaivibrio sulfuroxidans is TC8T (=DSM 101688T=JCM 31027T).

AB - A mesophilic, facultatively anaerobic, facultatively chemolithoautotrophic bacterium, designated strain TC8T, was isolated from a sulfidic shallow-water marine gas vent located at Tor Caldara, in the Tyrrhenian Sea, Italy. Cells were Gram-stain-negative curved rods with one or more polar flagella. Cells were approximately 1–1.5 μm in length and 0.6 μm in width. Strain TC8T grew between 20 and 35 °C (optimum 30 °C), with between 5 and 45 g NaCl l-1 (optimum 15–20 g l-1) and between pH 4.5 and 8.5 (optimum pH 6.0–7.0). The generation time under optimal conditions was 8 h. Strain TC8T was a facultative chemolithoautotroph also capable of using organic substrates as electron donors and carbon sources. Chemolithoautotrophic growth occurred with sulfur and thiosulfate as the electron donors, CO2 as the carbon source, and nitrate, oxygen (5 %, v/v) and ferric iron as the electron acceptors. Chemoorganoheterotrophic growth occurred with tryptone, peptone, Casamino acids, pyruvate and glycerol as substrates, while chemolithoherotrophic growth occurred with D(+)-glucose, sucrose, yeast extract, acetate, lactate, citrate and L-glutamine. The G+C content of the genomic DNA was 59.9 mol%. Phylogenetic analysis of the 16S rRNA gene sequence of strain TC8T showed that this organism formed a lineage within the family Rhodospirillaceae, which branched separately from the two closest relatives, Magnetovibrio blakemorei MV1T (91.25% similarity) and Magnetospira thiophila MMS-1T (90.13 %). Based on phylogenetic, physiological and chemotaxonomic characteristics, it is proposed that the organism represents a novel species of a new genus within the family Rhodospirillaceae, Varunaivibrio sulfuroxidans gen. nov., sp. nov. The type strain of Varunaivibrio sulfuroxidans is TC8T (=DSM 101688T=JCM 31027T).

KW - Alphaproteobacteria

KW - Chemosynthesis

KW - Gas vents

KW - Mediterranean Sea

KW - Mesophiles

KW - Nitrate

KW - Oxygen and iron respiration

KW - Sulfur-oxidation

KW - Tor Caldara

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U2 - 10.1099/ijsem.0.001235

DO - 10.1099/ijsem.0.001235

M3 - Article

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AN - SCOPUS:84991042637

VL - 66

SP - 3579

EP - 3584

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

SN - 1466-5026

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