TY - JOUR
T1 - VIP-, enkephalin-, substance P- and somatostatin-like immunoreactivity in neurons intrinsic to the intestine
T2 - immunohistochemical evidence from organotypic tissue cultures
AU - Schultzberg, Marianne
AU - Dreyfus, Cheryl F.
AU - Gershon, Michael D.
AU - Hökfelt, Tomas
AU - Elde, Robert P.
AU - Nilsson, Göran
AU - Said, Sami
AU - Goldstein, Menek
N1 - Funding Information:
This work was supported by grants from the Karolinska Institutet, The Swedish Medical Research Council (04X-2887, 04X-044945), Magnus Bergvalls Stiftelse, Sven och Ebba-Cristina Hagbergs Stiftelse, Harald och Greta Jeanssons Stiftelse, and by the following grants from the United States Department of Health, Education and Welfare: National Institutes of Health Grants HL 18994, HL-14187, NS 12969 and NS 06735, and Alcoholism, Drug Abuse and Mental Health Grants DA 01772, MH-02717 and MH-25504-02, and National Science Foundation Grant CH-8465. Cheryl Dreyfus is a fellow of the Pharmaceutical Manufacturers Association Foundation. The support of Professor Rolf Luft, Department of Endocrinology and Professor Bengt Pernow, Department of Clinical Physiology, Karolinska Hospital, as well as the generous supply of vasoactive intestinal polypeptide (VIP) by Professor Viktor Mutt, Department of Biochemistry, Karolinska Instituter, Stockholm, Sweden, is gratefully acknowledged. We also wish to thank Dr. Murray B. Bornstein of Albert Einstein College of Medicine for the use of his facilities. The skillful technical assistance of Mrs. W. Hiort is gratefully acknowledged.
PY - 1978/10/27
Y1 - 1978/10/27
N2 - Small intestine from 18-day fetal mice grown for 3 weeks in organotypic tissue culture was found to contain numerous VIP, enkephalin, substance P and some somatostatin immunoreactive nerve fibers. Since these cultures should be devoid of all afferent or other extrinsic neuronal inputs, it is concluded that there are VIP, enkephalin, substance P and somatostatin containing neurons intrinsic to the intestinal wall. However, all 4 peptides may also be present in neurons originating outside the gastrointestinal tract as well as in the intrinsic neurons.
AB - Small intestine from 18-day fetal mice grown for 3 weeks in organotypic tissue culture was found to contain numerous VIP, enkephalin, substance P and some somatostatin immunoreactive nerve fibers. Since these cultures should be devoid of all afferent or other extrinsic neuronal inputs, it is concluded that there are VIP, enkephalin, substance P and somatostatin containing neurons intrinsic to the intestinal wall. However, all 4 peptides may also be present in neurons originating outside the gastrointestinal tract as well as in the intrinsic neurons.
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U2 - 10.1016/0006-8993(78)91020-X
DO - 10.1016/0006-8993(78)91020-X
M3 - Article
AN - SCOPUS:0017808787
SN - 0006-8993
VL - 155
SP - 239
EP - 248
JO - Brain Research
JF - Brain Research
IS - 2
ER -