Vitamin A: Overlapping Delivery Pathways to Tissues from the Circulation

Jisun Paik, Silke Vogel, Loredana Quadro, Roseann Piantedosi, Max Gottesman, Katherine Lai, Leora Hamberger, Milena De Morais Vieira, William S. Blaner

Research output: Contribution to journalArticlepeer-review

50 Scopus citations

Abstract

Although retinol bound to retinol-binding protein (RBP) is the most abundant retinoid form present in the circulations of humans and most mammals, other retinoid and proretinoid forms are also present in the blood. We are interested in understanding to what extent each of these circulating retinoid forms contributes towards retinoid actions within cells and tissues. Here we report two studies focused on this question. First, we examined retinoid transport and storage in RBP-deficient mice that lack circulating RBP. These mice under normal laboratory conditions are phenotypically normal except for a visual impairment early in life that is corrected if the mice are maintained on a vitamin A-sufficient diet throughout life. The RBP-deficient mice take up vitamin A from the diet into most tissues at least as well as wild type mice. Compared to wild type mice, mice lacking RBP accumulate excess vitamin A in the liver, since there is no RBP to facilitate mobilization of stored retinol from hepatic stores. In a second study, we explored in vitro the actions of carotene cleavage enzyme (CCE) in facilitating β-carotene cleavage to retinoid in the testis. CCE is most highly expressed in the testis. Pull-down experiments coupled with MALDI-MS analysis showed that mouse testis CCE is able to interact with the testis-specific lactate dehydrogenase-C (LDH-C) isoform. This may suggest that CCE and LDH-C act in concert to catalyze β-carotene cleavage.

Original languageEnglish (US)
Pages (from-to)276S-280S
JournalJournal of Nutrition
Volume134
Issue number1
DOIs
StatePublished - Jan 2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Keywords

  • Carotene cleavage
  • Carotenoid
  • Chylomicron
  • Metabolism
  • Retinoid
  • Retinol-binding protein

Fingerprint Dive into the research topics of 'Vitamin A: Overlapping Delivery Pathways to Tissues from the Circulation'. Together they form a unique fingerprint.

Cite this