Wortmannin, an inhibitor of phosphatidylinositol kinases, blocks the MgATP-dependent recovery of Kir6.2/SUR2A channels

Lai Hua Xie, Makoto Takano, Masafumi Kakei, Midori Okamura, Akinori Noma

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Abstract

1. In order to investigate the mechanism underlying MgATP-dependent recovery of ATP-sensitive potassium (K(ATP)) channels, we expressed Kir6.2/SUR2A (inwardly rectifying K+ channel subunit/sulfonylurea receptor) or C-terminal-truncated Kir6.2 (Kir6.2ΔC26) in COS7 cells (Green monkey kidney cells), and carried out inside-out patch clamp experiments. 2. After patch excision in ATP-free internal solution, the activity of Kir6.2/SUR2A channels could be maximally recovered by the application of 5 mM MgATP. Subsequent application of 100 μM Ca2+ induced a rapid decay of Kir6.2/SUR2A activity to 11.6 ± 1.1% (mean ± S.E.M.) of the control level (Ca2+-induced run-down; n = 64). 3. MgATP (5 mM) recovered 99.4 ± 4.2% (n = 13) of the Ca2+-induced run-down. Protein kinase inhibitors such as W-7, H-7, H-8 and genistein did not inhibit this reaction. However, wortmannin, an inhibitor of phosphatidylinositol 3- and 4-kinases, blocked the MgATP-dependent recovery in a concentration-dependent manner; the magnitudes of recovery were 35.7 ± 7.2% (10 μM) and 4.3 ± 2.5% (100 μM) of the Ca2+-induced run-down. 4. MgUDP (10 mM) reversed the Ca2+-induced run-down of Kir6.2/XUR2A channels by 60.4 ± 7.6% (n = 5). Wortmannin failed to modify this reaction. 5. Kir6.2ΔC26 channels, which opened in the absence of SUR2A, were less sensitive to Ca2+; Kir6.2ΔC26 channels were inactivated to 44.8 ± 4.4% (n = 14) by 100 μM Ca2+. MgATP recovered the Ca2+-induced run-down of Kir6.2ΔC26 by 89.8 ± 7.7% (n = 9), and 100 μM wortmannin inhibited this reaction (1.8 ± 2%, n = 7). 6. Application of 10 μM phosphatidylinositol-4,5-bisphosphate (PI-4,5-P2) recovered the activity of Kir6.2/SUR2A channels after Ca2+-induced run-down (104.3 ± 6.4%, n = 10). Even after the MgATP-dependent recovery was blocked 100 μM wortmannin, PI-4,5-P2 reactivated the channels (102.3 ± 8.6%, n = 5). Similar results were obtained with Kir6.2ΔC26. 7. These results suggest that the entity of MgATP-dependent recovery may be membrane lipid phosphorylation rather than protein phosphorylation, and that synthesis of PI-4,5-P2 or phosphatidylinositol-3,4,5-trisphosphate may upregulate Kir6.2 channels.

Original languageEnglish (US)
Pages (from-to)655-665
Number of pages11
JournalJournal of Physiology
Volume514
Issue number3
DOIs
StatePublished - Feb 1 1999
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Physiology

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