YBR246W is required for the third step of diphthamide biosynthesis

Xiaoyang Su, Wei Chen, Wankyu Lee, Hong Jiang, Sheng Zhang, Hening Lin

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


Diphthamide, the target of diphtheria toxin, is a post-translationally modified histidine residue that is found in archaeal and eukaryotic translation elongation factor 2. The biosynthesis and function of this modification has attracted the interest of many biochemists for decades. The biosynthesis has been known to proceed in three steps. Proteins required for the first and second steps have been identified, but the protein(s) required for the last step have remained elusive. Here we demonstrate that the YBR246W gene in yeast is required for the last step of diphthamide biosynthesis, as the deletion of YBR246W leads to the accumulation of diphthine, which is the enzymatic product of the second step of the biosynthesis. This discovery will provide important information leading to the complete elucidation of the full biosynthesis pathway of diphthamide.

Original languageEnglish (US)
Pages (from-to)773-776
Number of pages4
JournalJournal of the American Chemical Society
Issue number2
StatePublished - Jan 18 2012
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry


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