Zn2+ depresses GABAA receptor mediated responses in acutely dissociated sacral dorsal commissural neurons

Zhi Ping Pang; Dian Shi Wang; Tian Le Xu; Ji Shuo Li

Zn²⁺ depresses GABA_A receptor mediated responses in acutely dissociated sacral dorsal commissural neurons

Zhi Ping Pang, Dian Shi Wang, Tian Le Xu, Ji Shuo Li

Research output: Contribution to journal › Article › peer-review

Abstract

The effects of Zn²⁺ on GABA_A-receptor mediated responses in acutely isolated rat sacral dorsal commissural nucleus (SDCN) were studied using nystatin-perforated whole cell recording techniques. The results demonstrated that (1) GABA induced inward currents through activation of GABA_A-receptor at a holding potential of - 40 mV; (2) GABA_A-receptor mediated responses were suppressed by Zn²⁺ in a reversible and voltage-independent manner; and (3) in the presence of Zn²⁺, the concentration-response curve of GABA-induced responses was shifted to the right in a parallel manner. The results suggest that Zn²⁺ allosterically depresses GABA_A-receptor mediated currents.

Original language	English (US)
Pages (from-to)	649-655
Number of pages	7
Journal	Acta Physiologica Sinica
Volume	50
Issue number	6
State	Published - 1998
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Medicine

Keywords

Dissociated cells
GABA-receptor
Nystatin-perforated whole cell recording
Sacral dorsal commissural nucleus
Zinc

Cite this

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title = "Zn2+ depresses GABAA receptor mediated responses in acutely dissociated sacral dorsal commissural neurons",

abstract = "The effects of Zn2+ on GABAA-receptor mediated responses in acutely isolated rat sacral dorsal commissural nucleus (SDCN) were studied using nystatin-perforated whole cell recording techniques. The results demonstrated that (1) GABA induced inward currents through activation of GABAA-receptor at a holding potential of - 40 mV; (2) GABAA-receptor mediated responses were suppressed by Zn2+ in a reversible and voltage-independent manner; and (3) in the presence of Zn2+, the concentration-response curve of GABA-induced responses was shifted to the right in a parallel manner. The results suggest that Zn2+ allosterically depresses GABAA-receptor mediated currents.",

keywords = "Dissociated cells, GABA-receptor, Nystatin-perforated whole cell recording, Sacral dorsal commissural nucleus, Zinc",

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T1 - Zn2+ depresses GABAA receptor mediated responses in acutely dissociated sacral dorsal commissural neurons

AU - Pang, Zhi Ping

AU - Wang, Dian Shi

AU - Xu, Tian Le

AU - Li, Ji Shuo

PY - 1998

Y1 - 1998

N2 - The effects of Zn2+ on GABAA-receptor mediated responses in acutely isolated rat sacral dorsal commissural nucleus (SDCN) were studied using nystatin-perforated whole cell recording techniques. The results demonstrated that (1) GABA induced inward currents through activation of GABAA-receptor at a holding potential of - 40 mV; (2) GABAA-receptor mediated responses were suppressed by Zn2+ in a reversible and voltage-independent manner; and (3) in the presence of Zn2+, the concentration-response curve of GABA-induced responses was shifted to the right in a parallel manner. The results suggest that Zn2+ allosterically depresses GABAA-receptor mediated currents.

AB - The effects of Zn2+ on GABAA-receptor mediated responses in acutely isolated rat sacral dorsal commissural nucleus (SDCN) were studied using nystatin-perforated whole cell recording techniques. The results demonstrated that (1) GABA induced inward currents through activation of GABAA-receptor at a holding potential of - 40 mV; (2) GABAA-receptor mediated responses were suppressed by Zn2+ in a reversible and voltage-independent manner; and (3) in the presence of Zn2+, the concentration-response curve of GABA-induced responses was shifted to the right in a parallel manner. The results suggest that Zn2+ allosterically depresses GABAA-receptor mediated currents.

KW - Dissociated cells

KW - GABA-receptor

KW - Nystatin-perforated whole cell recording

KW - Sacral dorsal commissural nucleus

KW - Zinc

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Zn²⁺ depresses GABA_A receptor mediated responses in acutely dissociated sacral dorsal commissural neurons

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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